Changes in the ganglioside long-chain base (LCB) composition in rat cerebellar granule cells in culture were studied during differentiation and aging. The total native ganglioside mixtures, extracted from the cells maintained in culture up to 22 days, were fractionated by reversed-phase HPLC, each ganglioside homogeneous in the oligosaccharide chain as well as in the LCB being quantified. Two main LCBs were components of the ganglioside species of cultured cells, the C18:1 LCB and the C20:1 LCB. The content of C20:1 ganglioside molecular species was low and quite constant during differentiation, comprising approximately 8% of the total ganglioside species content, the C20:1 LCB appearing to be represented more in the ganglioside of the "b series" (GD1b, GT1b, and GQ1b) than in the "a series" (GM1 and GD1a). During aging in culture, for 8-22 days, the content of the C20:1 species of all gangliosides increased, being more pronounced for GM1 and GD1a.

Valsecchi, M., Palestini, P., Chigorno, V., Sonnino, S., Tettamanti, G. (1993). Changes in the ganglioside long-chain base composition of rat cerebellar granule cells during differentiation and aging in culture. JOURNAL OF NEUROCHEMISTRY, 60(1), 193-196 [10.1111/j.1471-4159.1993.tb05837.x].

Changes in the ganglioside long-chain base composition of rat cerebellar granule cells during differentiation and aging in culture

PALESTINI, PAOLA NOVERINA ADA;
1993

Abstract

Changes in the ganglioside long-chain base (LCB) composition in rat cerebellar granule cells in culture were studied during differentiation and aging. The total native ganglioside mixtures, extracted from the cells maintained in culture up to 22 days, were fractionated by reversed-phase HPLC, each ganglioside homogeneous in the oligosaccharide chain as well as in the LCB being quantified. Two main LCBs were components of the ganglioside species of cultured cells, the C18:1 LCB and the C20:1 LCB. The content of C20:1 ganglioside molecular species was low and quite constant during differentiation, comprising approximately 8% of the total ganglioside species content, the C20:1 LCB appearing to be represented more in the ganglioside of the "b series" (GD1b, GT1b, and GQ1b) than in the "a series" (GM1 and GD1a). During aging in culture, for 8-22 days, the content of the C20:1 species of all gangliosides increased, being more pronounced for GM1 and GD1a.
Articolo in rivista - Articolo scientifico
Granulocytes; Cell Aging; Cerebellum; Cells, Cultured; Gangliosides; Chromatography, High Pressure Liquid; Cell Differentiation; Rats; Animals; Base Composition
English
193
196
Valsecchi, M., Palestini, P., Chigorno, V., Sonnino, S., Tettamanti, G. (1993). Changes in the ganglioside long-chain base composition of rat cerebellar granule cells during differentiation and aging in culture. JOURNAL OF NEUROCHEMISTRY, 60(1), 193-196 [10.1111/j.1471-4159.1993.tb05837.x].
Valsecchi, M; Palestini, P; Chigorno, V; Sonnino, S; Tettamanti, G
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/21430
Citazioni
  • Scopus 28
  • ???jsp.display-item.citation.isi??? 28
Social impact