Five major gangliosides (GM1, GD1a, GD1b, GT1b, and GQ1b) were extracted and isolated by normal-phase HPLC from the forebrain of Sprague-Dawley rats of ages ranging from 3 days to 24 months. Each ganglioside was fractionated by reverse-phase HPLC into the molecular species carrying a single long-chain base moiety. At all ages, the C18:1 and C20:1 long-chain base species predominated, whereas the C18:0 and C20:0 ones represented 1-3% of the total. The C18:1 long-chain base species, predominant at 3 days (91-96%), diminished with age and reached, at 2 years, 73%, 65%, 61%, 59%, and 45% of the total for GD1a, GM1, GT1b, GD1b, and GQ1b, respectively. The content of the C20:1 long-chain base species, low at birth (4-9%), increased with age in all gangliosides and reached, at 2 years, 27-55% of the total. The developmental behavior of the ganglioside species containing the C18:1 long-chain base was characterized by the following: (a) a biphasic profile with a maximum around 15 days for GD1a, the most abundant ganglioside at all ages; (b) an increase until 6 months for GM1; (c) a sharp decrease until 30 days, followed by leveling for GT1b; and (d) a low, constant level for GD1b and GQ1b. All the ganglioside species containing the C20:1 long-chain base showed a constant increase during development, the increase being more marked in the first 30 days.(ABSTRACT TRUNCATED AT 250 WORDS)

Palestini, P., Masserini, M., Sonnino, S., Giuliani, A., & Tettamanti, G. (1990). Changes in the ceramide composition of rat forebrain gangliosides with age. JOURNAL OF NEUROCHEMISTRY, 54(1), 230-235 [10.1111/j.1471-4159.1990.tb13305.x].

Changes in the Ceramide Composition of Rat Forebrain Gangliosides with Age

PALESTINI, PAOLA NOVERINA ADA;MASSERINI, MASSIMO ERNESTO;
1990

Abstract

Five major gangliosides (GM1, GD1a, GD1b, GT1b, and GQ1b) were extracted and isolated by normal-phase HPLC from the forebrain of Sprague-Dawley rats of ages ranging from 3 days to 24 months. Each ganglioside was fractionated by reverse-phase HPLC into the molecular species carrying a single long-chain base moiety. At all ages, the C18:1 and C20:1 long-chain base species predominated, whereas the C18:0 and C20:0 ones represented 1-3% of the total. The C18:1 long-chain base species, predominant at 3 days (91-96%), diminished with age and reached, at 2 years, 73%, 65%, 61%, 59%, and 45% of the total for GD1a, GM1, GT1b, GD1b, and GQ1b, respectively. The content of the C20:1 long-chain base species, low at birth (4-9%), increased with age in all gangliosides and reached, at 2 years, 27-55% of the total. The developmental behavior of the ganglioside species containing the C18:1 long-chain base was characterized by the following: (a) a biphasic profile with a maximum around 15 days for GD1a, the most abundant ganglioside at all ages; (b) an increase until 6 months for GM1; (c) a sharp decrease until 30 days, followed by leveling for GT1b; and (d) a low, constant level for GD1b and GQ1b. All the ganglioside species containing the C20:1 long-chain base showed a constant increase during development, the increase being more marked in the first 30 days.(ABSTRACT TRUNCATED AT 250 WORDS)
Articolo in rivista - Articolo scientifico
Ceramides; Chromatography, High Pressure Liquid; Rats; Animals; Brain Chemistry; Animals, Newborn; Gangliosides; Carbohydrate Conformation; Molecular Sequence Data; Brain; Carbohydrate Sequence; Fatty Acids; Aging
English
230
235
Palestini, P., Masserini, M., Sonnino, S., Giuliani, A., & Tettamanti, G. (1990). Changes in the ceramide composition of rat forebrain gangliosides with age. JOURNAL OF NEUROCHEMISTRY, 54(1), 230-235 [10.1111/j.1471-4159.1990.tb13305.x].
Palestini, P; Masserini, M; Sonnino, S; Giuliani, A; Tettamanti, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/21426
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