The current knowledge about the microbial communities associated with airborne particulate matter, particularly in urban areas, is limited. This study aims to fill this gap by describing the microbial community associated with coarse (PM10) and fine (PM2.5) particulate matter using pyrosequencing. Particulate matter was sampled on Teflon filters over 3 months in summer and 3 months in winter in Milan (Italy), and the hypervariable V3 region of the gene 16S rRNA amplified from the DNA extracted from the filters. The results showed large seasonal variations in the microbial communities, with plant-associated bacteria dominating in summer and spore-forming bacteria in winter. Bacterial communities from PM10 and PM2.5 were also found to differ from each other by season. In all samples, a high species richness, comparable with that of soils, but a low evenness was found. The results suggest that not only can the sources of the particulate influence the presence of specific bacterial groups but also that environmental factors and stresses can shape the bacterial community. © 2010 Springer-Verlag.

Franzetti, A., Gandolfi, I., Gaspari, E., Ambrosini, R., Bestetti, G. (2011). Seasonal variability of bacteria in fine and coarse urban air particulate matter. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(2), 745-753 [10.1007/s00253-010-3048-7].

Seasonal variability of bacteria in fine and coarse urban air particulate matter

FRANZETTI, ANDREA;GANDOLFI, ISABELLA;AMBROSINI, ROBERTO;BESTETTI, GIUSEPPINA
2011

Abstract

The current knowledge about the microbial communities associated with airborne particulate matter, particularly in urban areas, is limited. This study aims to fill this gap by describing the microbial community associated with coarse (PM10) and fine (PM2.5) particulate matter using pyrosequencing. Particulate matter was sampled on Teflon filters over 3 months in summer and 3 months in winter in Milan (Italy), and the hypervariable V3 region of the gene 16S rRNA amplified from the DNA extracted from the filters. The results showed large seasonal variations in the microbial communities, with plant-associated bacteria dominating in summer and spore-forming bacteria in winter. Bacterial communities from PM10 and PM2.5 were also found to differ from each other by season. In all samples, a high species richness, comparable with that of soils, but a low evenness was found. The results suggest that not only can the sources of the particulate influence the presence of specific bacterial groups but also that environmental factors and stresses can shape the bacterial community. © 2010 Springer-Verlag.
Articolo in rivista - Articolo scientifico
particulate matter, bioaerosol, bacterial community
English
2011
90
2
745
753
none
Franzetti, A., Gandolfi, I., Gaspari, E., Ambrosini, R., Bestetti, G. (2011). Seasonal variability of bacteria in fine and coarse urban air particulate matter. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 90(2), 745-753 [10.1007/s00253-010-3048-7].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/20480
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