Platelets release glutamate upon activation and are an important clearance system of the amino acid from blood, through high-affinity glutamate uptake, similar to that described in brain synaptosomes. Since platelet glutamate uptake is decreased in neurodegenerative disorders, we performed a morphological and molecular characterization of platelet glutamate transporters. The three major brain glutamate transporters EAAT1, EAAT2 and EAAT3 are expressed in platelets, with similar molecular weight, although at lower density than brain. A Na(+)-dependent-high-affinity glutamate uptake was competitively inhibited by known inhibitors but not by dihydrokainic acid, suggesting platelet EAAT2 does not play a major role in glutamate uptake at physiological conditions. We observed decreased glutamate uptake V(max), without modification of transporter affinity, in aging, which could be linked to the selective decrease of EAAT1 expression and mRNA. Moreover, in AD patients we found a further EAAT1 reduction compared to age-matched controls, which could explain the decrease of platelet uptake previously described. Platelet glutamate transporters may be used as peripheral markers to investigate the role of glutamate in patients with neuropsychiatric disorders.

Zoia, C., Cogliati, T., Tagliabue, E., Cavaletti, G., Sala, G., Galimberti, G., et al. (2004). Glutamate transporters in platelets: EAAT1 decrease in aging and in Alzheimer's disease. NEUROBIOLOGY OF AGING, 25(2), 149-157 [10.1016/S0197-4580(03)00085-X].

Glutamate transporters in platelets: EAAT1 decrease in aging and in Alzheimer's disease

ZOIA, CHIARA PAOLA;TAGLIABUE, ELENA;CAVALETTI, GUIDO ANGELO;SALA, GESSICA;GALIMBERTI, GLORIA;RIVOLTA, ILARIA;FERRARESE, CARLO
2004

Abstract

Platelets release glutamate upon activation and are an important clearance system of the amino acid from blood, through high-affinity glutamate uptake, similar to that described in brain synaptosomes. Since platelet glutamate uptake is decreased in neurodegenerative disorders, we performed a morphological and molecular characterization of platelet glutamate transporters. The three major brain glutamate transporters EAAT1, EAAT2 and EAAT3 are expressed in platelets, with similar molecular weight, although at lower density than brain. A Na(+)-dependent-high-affinity glutamate uptake was competitively inhibited by known inhibitors but not by dihydrokainic acid, suggesting platelet EAAT2 does not play a major role in glutamate uptake at physiological conditions. We observed decreased glutamate uptake V(max), without modification of transporter affinity, in aging, which could be linked to the selective decrease of EAAT1 expression and mRNA. Moreover, in AD patients we found a further EAAT1 reduction compared to age-matched controls, which could explain the decrease of platelet uptake previously described. Platelet glutamate transporters may be used as peripheral markers to investigate the role of glutamate in patients with neuropsychiatric disorders.
Articolo in rivista - Articolo scientifico
Blood Platelets; Middle Aged; Immunohistochemistry; Glutamate Plasma Membrane Transport Proteins; Blotting, Western; Excitatory Amino Acid Transporter 1; Glutamic Acid; Gene Expression; Amino Acid Transport System X-AG; Microscopy, Immunoelectron; Aged; Excitatory Amino Acid Transporter 2; Brain; Alzheimer Disease; Aged, 80 and over; Cell Line; Excitatory Amino Acid Transporter 3; Female; Humans; Symporters; RNA, Messenger; Reverse Transcriptase Polymerase Chain Reaction; Monocytes; Aging; Case-Control Studies
English
149
157
Zoia, C., Cogliati, T., Tagliabue, E., Cavaletti, G., Sala, G., Galimberti, G., et al. (2004). Glutamate transporters in platelets: EAAT1 decrease in aging and in Alzheimer's disease. NEUROBIOLOGY OF AGING, 25(2), 149-157 [10.1016/S0197-4580(03)00085-X].
Zoia, C; Cogliati, T; Tagliabue, E; Cavaletti, G; Sala, G; Galimberti, G; Rivolta, I; Rossi, V; Frattola, L; Ferrarese, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/20324
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