We used electrophoretic mobility shift assays to investigate the effects of cobalamin (Cbl) deficiency on the levels of activated nuclear factor-kappa B (NF-kappaB) in the spinal cords (SCs) and livers of rats made Cbl-deficient (Cbl-D) by total gastrectomy or a Cbl-D diet. We chose the SC and liver because they are severely or scarcely affected, respectively, by Cbl deficiency in terms of histological damage. We found permanently increased NF-kappaB levels (particularly the p50 and p65 subunits) in the SCs and livers of both types of Cbl-D rats, and Western blot analysis demonstrated increased p65 levels. NF-kappaB and p65 protein levels normalized when the totally gastrectomized (TGX) rats were treated with Cbl replacement. As we have previously demonstrated that Cbl deficiency increases tumor necrosis factor (TNF)-alpha and nerve growth factor (NGF) levels in the SC (each of which is a known NF-kappaB activator), we redetermined NF-kappaB levels in the SCs and livers of TGX rats treated with anti-TNF-alpha or anti-NGF antibodies and found that NF-kappaB levels normalized in both tissues after either treatment. These results demonstrate that: (1) Cbl physiologically and indirectly down-regulates NF-kappaB levels in rat SC and liver, and (2) NF-kappaB is an important signaling molecule after Cbl deficiency injury.

Veber, D., Mutti, E., Tacchini, L., Gammella, E., Tredici, G., Scalabrino, G. (2008). Indirect down-regulation of nuclear NF-kappaB levels by cobalamin in the spinal cord and liver of the rat. JOURNAL OF NEUROSCIENCE RESEARCH, 86(6), 1380-1387 [10.1002/jnr.21599].

Indirect down-regulation of nuclear NF-kappaB levels by cobalamin in the spinal cord and liver of the rat

TREDICI, GIOVANNI;
2008

Abstract

We used electrophoretic mobility shift assays to investigate the effects of cobalamin (Cbl) deficiency on the levels of activated nuclear factor-kappa B (NF-kappaB) in the spinal cords (SCs) and livers of rats made Cbl-deficient (Cbl-D) by total gastrectomy or a Cbl-D diet. We chose the SC and liver because they are severely or scarcely affected, respectively, by Cbl deficiency in terms of histological damage. We found permanently increased NF-kappaB levels (particularly the p50 and p65 subunits) in the SCs and livers of both types of Cbl-D rats, and Western blot analysis demonstrated increased p65 levels. NF-kappaB and p65 protein levels normalized when the totally gastrectomized (TGX) rats were treated with Cbl replacement. As we have previously demonstrated that Cbl deficiency increases tumor necrosis factor (TNF)-alpha and nerve growth factor (NGF) levels in the SC (each of which is a known NF-kappaB activator), we redetermined NF-kappaB levels in the SCs and livers of TGX rats treated with anti-TNF-alpha or anti-NGF antibodies and found that NF-kappaB levels normalized in both tissues after either treatment. These results demonstrate that: (1) Cbl physiologically and indirectly down-regulates NF-kappaB levels in rat SC and liver, and (2) NF-kappaB is an important signaling molecule after Cbl deficiency injury.
Articolo in rivista - Articolo scientifico
Cell Nucleus; Electrophoretic Mobility Shift Assay; Male; Spinal Cord; Vitamin B 12; Vitamin B 12 Deficiency; Gastrectomy; Rats; Animals; Down-Regulation; Synaptotagmin I; Blotting, Western; Rats, Sprague-Dawley; Liver; NF-kappa B
English
2008
86
6
1380
1387
none
Veber, D., Mutti, E., Tacchini, L., Gammella, E., Tredici, G., Scalabrino, G. (2008). Indirect down-regulation of nuclear NF-kappaB levels by cobalamin in the spinal cord and liver of the rat. JOURNAL OF NEUROSCIENCE RESEARCH, 86(6), 1380-1387 [10.1002/jnr.21599].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/20316
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