Integrin adhesion receptors transduce signals that control complex cell functions which require the regulation of gene expression, such as proliferation, differentiation and survival. Their intracellular domain has no catalytic function, indicating that interaction with other transducing molecules is crucial for integrin-mediated signalling. Here we have identified a protein that interacts with the cytoplasmic domain of the beta2 subunit of the alphaL/beta2 integrin LFA-1. This protein is JAB1 (Jun activation domain-binding protein 1), a coactivator of the c-Jun transcription factor. We found that JAB1 is present both in the nucleus and in the cytoplasm of cells and that a fraction of JAB1 colocalizes with LFA-1 at the cell membrane. LFA-1 engagement is followed by an increase of the nuclear pool of JAB1, paralleled by enhanced binding of c-Jun-containing AP-1 complexes to their DNA consensus site and increased transactivation of an AP-1-dependent promoter. We suggest that signalling through the LFA-1 integrin may affect c-Jun-driven transcription by regulating JAB1 nuclear localization. This represents a new pathway for integrin-dependent modulation of gene expression.

Bianchi, E., Denti, S., Granata, A., Bossi, G., Geginat, J., Villa, A., et al. (2000). Integrin LFA-1 interacts with the transcriptional co-activator JAB1 to modulate AP-1 activity. NATURE, 404(6778), 617-621 [10.1038/35007098].

Integrin LFA-1 interacts with the transcriptional co-activator JAB1 to modulate AP-1 activity

VILLA, ANTONELLO;
2000

Abstract

Integrin adhesion receptors transduce signals that control complex cell functions which require the regulation of gene expression, such as proliferation, differentiation and survival. Their intracellular domain has no catalytic function, indicating that interaction with other transducing molecules is crucial for integrin-mediated signalling. Here we have identified a protein that interacts with the cytoplasmic domain of the beta2 subunit of the alphaL/beta2 integrin LFA-1. This protein is JAB1 (Jun activation domain-binding protein 1), a coactivator of the c-Jun transcription factor. We found that JAB1 is present both in the nucleus and in the cytoplasm of cells and that a fraction of JAB1 colocalizes with LFA-1 at the cell membrane. LFA-1 engagement is followed by an increase of the nuclear pool of JAB1, paralleled by enhanced binding of c-Jun-containing AP-1 complexes to their DNA consensus site and increased transactivation of an AP-1-dependent promoter. We suggest that signalling through the LFA-1 integrin may affect c-Jun-driven transcription by regulating JAB1 nuclear localization. This represents a new pathway for integrin-dependent modulation of gene expression.
Articolo in rivista - Articolo scientifico
Cell Nucleus; Receptor Aggregation; Animals; T-Lymphocytes; DNA-Binding Proteins; Humans; COS Cells; Intracellular Signaling Peptides and Proteins; Signal Transduction; Gene Expression Regulation; Transcription Factors; Peptide Hydrolases; Lymphocyte Function-Associated Antigen-1; Transcription, Genetic; Transcription Factor AP-1; Antibodies, Monoclonal; Luciferases; Protein Binding; Cytoplasm; Jurkat Cells
English
6-apr-2000
404
6778
617
621
none
Bianchi, E., Denti, S., Granata, A., Bossi, G., Geginat, J., Villa, A., et al. (2000). Integrin LFA-1 interacts with the transcriptional co-activator JAB1 to modulate AP-1 activity. NATURE, 404(6778), 617-621 [10.1038/35007098].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/19408
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