Agrin controls the formation of the neuromuscular junction. Whether it regulates the differentiation of other types of synapses remains unclear. Therefore, we have studied the role of agrin in cultured hippocampal neurons. Synaptogenesis was severely compromised when agrin expression or function was suppressed by antisense oligonucleotides and specific antibodies. The effects of antisense oligonucleotides were found to be highly specific because they were reversed by adding recombinant agrin and could not be detected in cultures from agrin-deficient animals. Interestingly, the few synapses formed in reduced agrin conditions displayed diminished vesicular turnover, despite a normal appearance at the EM level. Thus, our results demonstrate the necessity of agrin for synaptogenesis in hippocampal neurons.

Bose, C., Qiu, D., Bergamaschi, A., Gravante, B., Bossi, M., Villa, A., et al. (2000). Agrin controls synaptic differentiation in hippocampal neurons. THE JOURNAL OF NEUROSCIENCE, 20(24), 9086-9095.

Agrin controls synaptic differentiation in hippocampal neurons

BOSSI, MARIO;VILLA, ANTONELLO;
2000

Abstract

Agrin controls the formation of the neuromuscular junction. Whether it regulates the differentiation of other types of synapses remains unclear. Therefore, we have studied the role of agrin in cultured hippocampal neurons. Synaptogenesis was severely compromised when agrin expression or function was suppressed by antisense oligonucleotides and specific antibodies. The effects of antisense oligonucleotides were found to be highly specific because they were reversed by adding recombinant agrin and could not be detected in cultures from agrin-deficient animals. Interestingly, the few synapses formed in reduced agrin conditions displayed diminished vesicular turnover, despite a normal appearance at the EM level. Thus, our results demonstrate the necessity of agrin for synaptogenesis in hippocampal neurons.
Articolo in rivista - Articolo scientifico
Hippocampus; Cells, Cultured; Synapses; Antibodies; Endocytosis; Rats; Animals; Oligonucleotides, Antisense; Receptors, N-Methyl-D-Aspartate; Rats, Sprague-Dawley; Patch-Clamp Techniques; Electric Stimulation; Synaptic Vesicles; Cell Count; Tetrodotoxin; Agrin; Evoked Potentials; Exocytosis; Neurons; Recombinant Proteins; Cell Differentiation; Synaptic Transmission
English
9086
9095
Bose, C., Qiu, D., Bergamaschi, A., Gravante, B., Bossi, M., Villa, A., et al. (2000). Agrin controls synaptic differentiation in hippocampal neurons. THE JOURNAL OF NEUROSCIENCE, 20(24), 9086-9095.
Bose, C; Qiu, D; Bergamaschi, A; Gravante, B; Bossi, M; Villa, A; Rupp, F; Malgaroli, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/19405
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