The yield obtained in a Candida rugosa lipase-catalysed heptyl acetate synthesis via transesterification of 1-heptanol with vinyl acetate depends linearly on the concentration of lipases of each crude lyophilised powder. Thus, it is possible to characterise the amount of lipases present in the biocatalyst by calculating the parameter called Catalytic Performance (CP), defined as (% final yield)×(mg crude biocatalyst)¿1. On the other hand, the relative yield obtained in the C. rugosa lipase-catalysed transesterifications of different alcohols (1-heptanol, geraniol, nerol and cyclohexanol) with vinyl acetate as acyl donor depends on the proportion of isoenzymes of each crude biocatalyst. Therefore, it is possible to qualitatively evaluate the proportion of isoenzymes in those crude preparations and to predict the biocatalytical behaviour of each isoenzyme according to the alcohol employed. The methodology described is successfully used in two non-conventional asymmetric syntheses in organic media.

Dominguez de Maria, P., Sinisterra, J., Sanchez Montero, J., Lotti, M., Valero, F., & Alcantara, A. (2006). Acyl transfer strategy for the biocatalytical characterisation of Candida rugosa lipases in organic solvents. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 38(1-2), 199-208 [10.1016/j.enzmictec.2005.06.001].

Acyl transfer strategy for the biocatalytical characterisation of Candida rugosa lipases in organic solvents

LOTTI, MARINA;
2006

Abstract

The yield obtained in a Candida rugosa lipase-catalysed heptyl acetate synthesis via transesterification of 1-heptanol with vinyl acetate depends linearly on the concentration of lipases of each crude lyophilised powder. Thus, it is possible to characterise the amount of lipases present in the biocatalyst by calculating the parameter called Catalytic Performance (CP), defined as (% final yield)×(mg crude biocatalyst)¿1. On the other hand, the relative yield obtained in the C. rugosa lipase-catalysed transesterifications of different alcohols (1-heptanol, geraniol, nerol and cyclohexanol) with vinyl acetate as acyl donor depends on the proportion of isoenzymes of each crude biocatalyst. Therefore, it is possible to qualitatively evaluate the proportion of isoenzymes in those crude preparations and to predict the biocatalytical behaviour of each isoenzyme according to the alcohol employed. The methodology described is successfully used in two non-conventional asymmetric syntheses in organic media.
Articolo in rivista - Articolo scientifico
Candida rugosa lipases; Isoenzymes; Acyl transfer; Catalytic performance; Organic solvents
English
199
208
Dominguez de Maria, P., Sinisterra, J., Sanchez Montero, J., Lotti, M., Valero, F., & Alcantara, A. (2006). Acyl transfer strategy for the biocatalytical characterisation of Candida rugosa lipases in organic solvents. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC, 38(1-2), 199-208 [10.1016/j.enzmictec.2005.06.001].
Dominguez de Maria, P; Sinisterra, J; Sanchez Montero, J; Lotti, M; Valero, F; Alcantara, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/1934
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