A method of freezing fixed surgical tissue specimens for long-term storage and subsequent resin embedding was developed. The method entails fixation of the specimens in Karnowsky's liquid, cryoprotecting with 10% sucrose, freezing in melting Freon gas, and storage at -80°C. When needed, even after years, the frozen tissue pieces are thawed in lukewarm (37°C) buffer and embedded in resin (Epon or Lowicryl). Ultrastructural detail as well as immunocytochemical reactivity of a wide variety of cellular constituents are well preserved. The method is practical and allows retrospective embedding in Lowicryl resin (a technique not easily applicable in everyday routine) for subsequent ultrastructural immunolocalization studies. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted
Scopsi, L., Migliari, M., Vergani, B., Lombardi, L. (1992). New Techniques:The Use of Fixed-Frozen Tissue in Routine Ultrastructural Evaluation and Immunogold Labeling. ULTRASTRUCTURAL PATHOLOGY, 16(3), 351-362 [10.3109/01913129209061363].
New Techniques:The Use of Fixed-Frozen Tissue in Routine Ultrastructural Evaluation and Immunogold Labeling
Vergani, B;
1992
Abstract
A method of freezing fixed surgical tissue specimens for long-term storage and subsequent resin embedding was developed. The method entails fixation of the specimens in Karnowsky's liquid, cryoprotecting with 10% sucrose, freezing in melting Freon gas, and storage at -80°C. When needed, even after years, the frozen tissue pieces are thawed in lukewarm (37°C) buffer and embedded in resin (Epon or Lowicryl). Ultrastructural detail as well as immunocytochemical reactivity of a wide variety of cellular constituents are well preserved. The method is practical and allows retrospective embedding in Lowicryl resin (a technique not easily applicable in everyday routine) for subsequent ultrastructural immunolocalization studies. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permittedI documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.