A method of freezing fixed surgical tissue specimens for long-term storage and subsequent resin embedding was developed. The method entails fixation of the specimens in Karnowsky's liquid, cryoprotecting with 10% sucrose, freezing in melting Freon gas, and storage at -80°C. When needed, even after years, the frozen tissue pieces are thawed in lukewarm (37°C) buffer and embedded in resin (Epon or Lowicryl). Ultrastructural detail as well as immunocytochemical reactivity of a wide variety of cellular constituents are well preserved. The method is practical and allows retrospective embedding in Lowicryl resin (a technique not easily applicable in everyday routine) for subsequent ultrastructural immunolocalization studies. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted

Scopsi, L., Migliari, M., Vergani, B., Lombardi, L. (1992). New Techniques:The Use of Fixed-Frozen Tissue in Routine Ultrastructural Evaluation and Immunogold Labeling. ULTRASTRUCTURAL PATHOLOGY, 16(3), 351-362 [10.3109/01913129209061363].

New Techniques:The Use of Fixed-Frozen Tissue in Routine Ultrastructural Evaluation and Immunogold Labeling

Vergani, B;
1992

Abstract

A method of freezing fixed surgical tissue specimens for long-term storage and subsequent resin embedding was developed. The method entails fixation of the specimens in Karnowsky's liquid, cryoprotecting with 10% sucrose, freezing in melting Freon gas, and storage at -80°C. When needed, even after years, the frozen tissue pieces are thawed in lukewarm (37°C) buffer and embedded in resin (Epon or Lowicryl). Ultrastructural detail as well as immunocytochemical reactivity of a wide variety of cellular constituents are well preserved. The method is practical and allows retrospective embedding in Lowicryl resin (a technique not easily applicable in everyday routine) for subsequent ultrastructural immunolocalization studies. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted
Articolo in rivista - Articolo scientifico
Freeze-thawing Immunogold labeling Long-term tissue storage Ultrastructural preservation
English
1992
16
3
351
362
none
Scopsi, L., Migliari, M., Vergani, B., Lombardi, L. (1992). New Techniques:The Use of Fixed-Frozen Tissue in Routine Ultrastructural Evaluation and Immunogold Labeling. ULTRASTRUCTURAL PATHOLOGY, 16(3), 351-362 [10.3109/01913129209061363].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/191095
Citazioni
  • Scopus 8
  • ???jsp.display-item.citation.isi??? 10
Social impact