The binding of 125I-labeled substance P (SP) to rat brain cortex membranes has been studied under control conditions and in the presence of ethanol. The binding of SP at low concentrations (20-1000 pM) gave two components, one with a KD value of 80 pM and another one with a KD of 500 pM. The higher-affinity component is due to NK1 receptors, as confirmed by the inhibition of the SP binding by the rodent NK1 specific agonist [Sar9 Met(O2)11]SP. Ethanol (1.7 mM) added to the binding assays inhibited by more than 50% the specific binding at a very low SP concentration (20 pM); however, it had no effect at SP concentrations ranging from 50 to 120 pM. This suggests a decrease by ethanol of the affinity of SP to the NK1 receptors involved in this binding component. The ethanol effect disappeared at [EtOH] < or = 0.17 mM
Scarrone, S., Cappelli, A., Cupello, A., Matarrese, M., Moresco, R., & Fazio, F. (2003). Ethanol Inhibits the Binding of Substance P to Rat Brain Cortex NK1 Receptors. NEUROCHEMICAL RESEARCH, 28(8), 1159-1162.
Citazione: | Scarrone, S., Cappelli, A., Cupello, A., Matarrese, M., Moresco, R., & Fazio, F. (2003). Ethanol Inhibits the Binding of Substance P to Rat Brain Cortex NK1 Receptors. NEUROCHEMICAL RESEARCH, 28(8), 1159-1162. |
Tipo: | Articolo in rivista - Articolo scientifico |
Carattere della pubblicazione: | Scientifica |
Presenza di un coautore afferente ad Istituzioni straniere: | No |
Titolo: | Ethanol Inhibits the Binding of Substance P to Rat Brain Cortex NK1 Receptors |
Autori: | Scarrone, S; Cappelli, A; Cupello, A; Matarrese, M; Moresco, R; Fazio, F |
Autori: | |
Data di pubblicazione: | 2003 |
Lingua: | English |
Rivista: | NEUROCHEMICAL RESEARCH |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1023/A:1024216109472 |
Appare nelle tipologie: | 01 - Articolo su rivista |