Long-term potentiation has previously been studied with electrophysiological techniques that do not readily separate presynaptic and postsynaptic contributions. Changes in exocytotic-endocytotic cycling have now been monitored at synapses between cultured rat hippocampal neurons by measuring the differential uptake of antibodies that recognize the intraluminal domain of the synaptic vesicle protein synaptotagmin. Vesicular cycling increased markedly during glutamate-induced long-term potentiation. The degree of potentiation was heterogeneous, appearing greater at synapses at which the initial extent of vesicular turnover was low. Thus, changes in presynaptic activity were visualized directly and the spatial distribution of potentiation could be determined at the level of single synaptic boutons.

Malgaroli, A., Ting, A., Wendland, B., Bergamaschi, A., Villa, A., Tsien, R., et al. (1995). Presynaptic component of long-term potentiation visualized at individual hippocampal synapses. SCIENCE, 268(5217), 1624-1628 [10.1126/science.7777862].

Presynaptic component of long-term potentiation visualized at individual hippocampal synapses

VILLA, ANTONELLO;
1995

Abstract

Long-term potentiation has previously been studied with electrophysiological techniques that do not readily separate presynaptic and postsynaptic contributions. Changes in exocytotic-endocytotic cycling have now been monitored at synapses between cultured rat hippocampal neurons by measuring the differential uptake of antibodies that recognize the intraluminal domain of the synaptic vesicle protein synaptotagmin. Vesicular cycling increased markedly during glutamate-induced long-term potentiation. The degree of potentiation was heterogeneous, appearing greater at synapses at which the initial extent of vesicular turnover was low. Thus, changes in presynaptic activity were visualized directly and the spatial distribution of potentiation could be determined at the level of single synaptic boutons.
Articolo in rivista - Articolo scientifico
Hippocampus; Membrane Glycoproteins; Calcium-Binding Proteins; Cells, Cultured; Synaptotagmins; Rats; Animals; Receptors, N-Methyl-D-Aspartate; Patch-Clamp Techniques; Glutamic Acid; Synaptic Vesicles; Potassium; Neurons; Nerve Tissue Proteins; Presynaptic Terminals; Long-Term Potentiation; Molecular Sequence Data; Amino Acid Sequence; Pyrroles; Synaptic Transmission
English
16-giu-1995
268
5217
1624
1628
none
Malgaroli, A., Ting, A., Wendland, B., Bergamaschi, A., Villa, A., Tsien, R., et al. (1995). Presynaptic component of long-term potentiation visualized at individual hippocampal synapses. SCIENCE, 268(5217), 1624-1628 [10.1126/science.7777862].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/18445
Citazioni
  • Scopus 164
  • ???jsp.display-item.citation.isi??? 150
Social impact