The adoption of high-througput technologies demonstrated that in mature spermatozoa are present proteins that are thought to be not present or active in sperm cells, such as those involved in control of cell cycle. Here, by using an in silico approach based on the application of networks theory, we found that Cyclins/Cdk complexes could play a central role in signal transduction active during capacitation. Then, we tested this hypothesis in the vitro model. With this approach, spermatozoa were incubated under capacitating conditions in control conditions (CTRL) or in the presence of Aminopurvalanol A a potent, selective and cell permeable inhibitor of Cyclins/Cdk complexes at different concentrations (2, 10, and 20 μM). We found that this treatment caused dose-dependent inhibition of sperm fertilizing ability. We attribute this event to the loss of acrosome integrity due to the inhibition of physiological capacitation-dependent actin polymerization, rather than to a detrimental effect on membrane lipid remodeling or on other signaling pathways such as tubulin reorganization or MAPKs activation. In our opinion, these data could revamp the knowledge on biochemistry of sperm capacitation and could suggest new perspectives in studying male infertility.

Bernabã², N., Valbonetti, L., Greco, L., Capacchietti, G., Sanchez, M., Palestini, P., et al. (2017). Aminopurvalanol A, a potent, selective, and cell permeable inhibitor of Cyclins/Cdk complexes, causes the reduction of in vitro fertilizing ability of boar spermatozoa, by negatively affecting the capacitation-dependent actin polymerization. FRONTIERS IN PHYSIOLOGY, 8(DEC), 1-16 [10.3389/fphys.2017.01097].

Aminopurvalanol A, a potent, selective, and cell permeable inhibitor of Cyclins/Cdk complexes, causes the reduction of in vitro fertilizing ability of boar spermatozoa, by negatively affecting the capacitation-dependent actin polymerization

Palestini, P;Botto, L;
2017

Abstract

The adoption of high-througput technologies demonstrated that in mature spermatozoa are present proteins that are thought to be not present or active in sperm cells, such as those involved in control of cell cycle. Here, by using an in silico approach based on the application of networks theory, we found that Cyclins/Cdk complexes could play a central role in signal transduction active during capacitation. Then, we tested this hypothesis in the vitro model. With this approach, spermatozoa were incubated under capacitating conditions in control conditions (CTRL) or in the presence of Aminopurvalanol A a potent, selective and cell permeable inhibitor of Cyclins/Cdk complexes at different concentrations (2, 10, and 20 μM). We found that this treatment caused dose-dependent inhibition of sperm fertilizing ability. We attribute this event to the loss of acrosome integrity due to the inhibition of physiological capacitation-dependent actin polymerization, rather than to a detrimental effect on membrane lipid remodeling or on other signaling pathways such as tubulin reorganization or MAPKs activation. In our opinion, these data could revamp the knowledge on biochemistry of sperm capacitation and could suggest new perspectives in studying male infertility.
Articolo in rivista - Articolo scientifico
Acrosome reaction; Actin; Aminopurvalanol A; Cell cycle; Cyclins/Cdk complexes; Cytoskeleton; In vitro fertilization; Sperm capacitation;
Acrosome reaction; Actin; Aminopurvalanol A; Cell cycle; Cyclins/Cdk complexes; Cytoskeleton; In vitro fertilization; Sperm capacitation; Physiology; Physiology (medical)
English
dic-2017
2017
8
DEC
1
16
1097
open
Bernabã², N., Valbonetti, L., Greco, L., Capacchietti, G., Sanchez, M., Palestini, P., et al. (2017). Aminopurvalanol A, a potent, selective, and cell permeable inhibitor of Cyclins/Cdk complexes, causes the reduction of in vitro fertilizing ability of boar spermatozoa, by negatively affecting the capacitation-dependent actin polymerization. FRONTIERS IN PHYSIOLOGY, 8(DEC), 1-16 [10.3389/fphys.2017.01097].
File in questo prodotto:
File Dimensione Formato  
75 Frontiers Teramo 17.pdf

accesso aperto

Dimensione 2.83 MB
Formato Adobe PDF
2.83 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/182383
Citazioni
  • Scopus 6
  • ???jsp.display-item.citation.isi??? 7
Social impact