Objectives We have previously shown the importance of the complement system in differentiating between patients with squamous cell carcinoma of the penis (SCCP) and controls. These patients had low expression of C3a and C4 fragments. Therefore, in this study, we investigated the complement protein C3a as a potential circulating biomarker in these patients by a commercially available enzyme-linked immunosorbent assay (ELISA) test. Patients and methods Plasma samples from 39 patients with SCCP, 15 patients with prostate cancer, and 50 healthy male subjects were evaluated using the ELISA—Bioscience OptEIA Kit human anti-C3a (BD). The nonparametric Mann-Whitney test was used for comparison of means among the groups. Results The complement protein C3a was found down expressed in patients with SCCP (P<0.05) in comparison to either subjects with good health or subjects with prostate cancer. Conclusion Experimental validation of the down expression of C3a was well succeeded using a commercial ELISA kit. Complement system fragment C3a is down expressed in patients with SCCP. Besides, C3a is also low expressed in the plasma of patients with initial prostate cancer when compared to healthy subjects. These results suggest that the innate immune response might be suppressed in patients with these malignancies.

Ornellas, P., Ribeiro Carvalho, M., Ornellas, A., Scherrer, L., Koifman, L., Magni, F., et al. (2017). Experimental validation of the complement protein C3a down expression in the plasma of patients with squamous cell carcinoma of the penis. UROLOGIC ONCOLOGY, 35(9), 545.e13-545.e18 [10.1016/j.urolonc.2017.05.002].

Experimental validation of the complement protein C3a down expression in the plasma of patients with squamous cell carcinoma of the penis

Magni, F;
2017

Abstract

Objectives We have previously shown the importance of the complement system in differentiating between patients with squamous cell carcinoma of the penis (SCCP) and controls. These patients had low expression of C3a and C4 fragments. Therefore, in this study, we investigated the complement protein C3a as a potential circulating biomarker in these patients by a commercially available enzyme-linked immunosorbent assay (ELISA) test. Patients and methods Plasma samples from 39 patients with SCCP, 15 patients with prostate cancer, and 50 healthy male subjects were evaluated using the ELISA—Bioscience OptEIA Kit human anti-C3a (BD). The nonparametric Mann-Whitney test was used for comparison of means among the groups. Results The complement protein C3a was found down expressed in patients with SCCP (P<0.05) in comparison to either subjects with good health or subjects with prostate cancer. Conclusion Experimental validation of the down expression of C3a was well succeeded using a commercial ELISA kit. Complement system fragment C3a is down expressed in patients with SCCP. Besides, C3a is also low expressed in the plasma of patients with initial prostate cancer when compared to healthy subjects. These results suggest that the innate immune response might be suppressed in patients with these malignancies.
Articolo in rivista - Articolo scientifico
Circulating biomarker; Enzyme-linked immunosorbent assay (ELISA); Human complement C3a-desArg/C4a-desArg; Penile cancer; Plasma; Tumor microenvironment
English
25-mag-2017
2017
35
9
545.e13
545.e18
none
Ornellas, P., Ribeiro Carvalho, M., Ornellas, A., Scherrer, L., Koifman, L., Magni, F., et al. (2017). Experimental validation of the complement protein C3a down expression in the plasma of patients with squamous cell carcinoma of the penis. UROLOGIC ONCOLOGY, 35(9), 545.e13-545.e18 [10.1016/j.urolonc.2017.05.002].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/181573
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