Second Harmonic Generation (SHG) is a label-free imaging method used to monitor collagen organization in tissues. Due to its sensitivity to the incident polarization, it provides microstructural information otherwise unreachable by other intensity based imaging methods. We develop and test a Microscopic Multiparametric Analysis by Phasor projection of Polarization-dependent SHG (μMAPPS) that maps the features of the collagen architecture in tissues at the micrometer scale. μMAPPS retrieves pixel-by-pixel the collagen fibrils anisotropy and orientation by operating directly on two coupled phasor spaces, avoiding direct fitting of the polarization dependent SHG signal. We apply μMAPPS to fixed tissue sections and to the study of the collagen microscopic organization in tumors ex-vivo and in-vivo. We develop a clustering algorithm to automatically group pixels with similar microstructural features. μMAPPS can perform fast analyses of tissues and opens to future applications for in-situ diagnosis of pathologies and diseases that could assist histo-pathological evaluation

Radaelli, F., D'Alfonso, L., Collini, M., Mingozzi, F., Marongiu, L., Granucci, F., et al. (2017). μMAPPS: A novel phasor approach to second harmonic analysis for in vitro-in vivo investigation of collagen microstructure. SCIENTIFIC REPORTS, 7(1) [10.1038/s41598-017-17726-y].

μMAPPS: A novel phasor approach to second harmonic analysis for in vitro-in vivo investigation of collagen microstructure

D'Alfonso, L.;Collini, M.
;
Mingozzi, F.;Marongiu, L.;Granucci, F.;Zanoni, I.;Chirico, G.;Sironi, L.
Ultimo
2017

Abstract

Second Harmonic Generation (SHG) is a label-free imaging method used to monitor collagen organization in tissues. Due to its sensitivity to the incident polarization, it provides microstructural information otherwise unreachable by other intensity based imaging methods. We develop and test a Microscopic Multiparametric Analysis by Phasor projection of Polarization-dependent SHG (μMAPPS) that maps the features of the collagen architecture in tissues at the micrometer scale. μMAPPS retrieves pixel-by-pixel the collagen fibrils anisotropy and orientation by operating directly on two coupled phasor spaces, avoiding direct fitting of the polarization dependent SHG signal. We apply μMAPPS to fixed tissue sections and to the study of the collagen microscopic organization in tumors ex-vivo and in-vivo. We develop a clustering algorithm to automatically group pixels with similar microstructural features. μMAPPS can perform fast analyses of tissues and opens to future applications for in-situ diagnosis of pathologies and diseases that could assist histo-pathological evaluation
Articolo in rivista - Articolo scientifico
Second Harmonic Generation, tumor, collagen, phasor analysis, clustering algorithms
English
Radaelli, F., D'Alfonso, L., Collini, M., Mingozzi, F., Marongiu, L., Granucci, F., et al. (2017). μMAPPS: A novel phasor approach to second harmonic analysis for in vitro-in vivo investigation of collagen microstructure. SCIENTIFIC REPORTS, 7(1) [10.1038/s41598-017-17726-y].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/179846
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