The three-dimensional (3D) structure of the catalytic domain of Gas1p, a protein belonging to the only family of beta-(1,3)-glucan transferases so far identified in yeasts and some pathogenic fungi (family GH-72), has been predicted by combining results derived from threading methods, multiple sequence alignments and secondary-structure predictions. The 3D model has allowed the identification of several residues that are predicted to play a crucial role in structural integrity, substrate recognition and catalysis. In particular, the model of the catalytic domain can be useful for designing site-directed mutagenesis experiments and for developing inhibitors of Gas1p enzymatic activity.
Papaleo, E., Fantucci, P., Vai, M., & De Gioia, L. (2006). Three-dimensional structure of the catalytic domain of the yeast beta-(1,3)-glucan transferase Gas1: a molecular modeling investigation. JOURNAL OF MOLECULAR MODELING, 12(2), 237-248 [10.1007/s00894-005-0025-7].
Citazione: | Papaleo, E., Fantucci, P., Vai, M., & De Gioia, L. (2006). Three-dimensional structure of the catalytic domain of the yeast beta-(1,3)-glucan transferase Gas1: a molecular modeling investigation. JOURNAL OF MOLECULAR MODELING, 12(2), 237-248 [10.1007/s00894-005-0025-7]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Titolo: | Three-dimensional structure of the catalytic domain of the yeast beta-(1,3)-glucan transferase Gas1: a molecular modeling investigation | |
Autori: | Papaleo, E; Fantucci, P; Vai, M; De Gioia, L | |
Autori: | ||
Data di pubblicazione: | gen-2006 | |
Lingua: | English | |
Rivista: | JOURNAL OF MOLECULAR MODELING | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1007/s00894-005-0025-7 | |
Appare nelle tipologie: | 01 - Articolo su rivista |