The three-dimensional (3D) structure of the catalytic domain of Gas1p, a protein belonging to the only family of beta-(1,3)-glucan transferases so far identified in yeasts and some pathogenic fungi (family GH-72), has been predicted by combining results derived from threading methods, multiple sequence alignments and secondary-structure predictions. The 3D model has allowed the identification of several residues that are predicted to play a crucial role in structural integrity, substrate recognition and catalysis. In particular, the model of the catalytic domain can be useful for designing site-directed mutagenesis experiments and for developing inhibitors of Gas1p enzymatic activity.

Papaleo, E., Fantucci, P., Vai, M., & DE GIOIA, L. (2006). Three-dimensional structure of the catalytic domain of the yeast beta-(1,3)-glucan transferase Gas1: a molecular modeling investigation. JOURNAL OF MOLECULAR MODELING, 12(2), 237-248 [10.1007/s00894-005-0025-7].

Three-dimensional structure of the catalytic domain of the yeast beta-(1,3)-glucan transferase Gas1: a molecular modeling investigation

FANTUCCI, PIERCARLO;VAI, MARINA;DE GIOIA, LUCA
2006

Abstract

The three-dimensional (3D) structure of the catalytic domain of Gas1p, a protein belonging to the only family of beta-(1,3)-glucan transferases so far identified in yeasts and some pathogenic fungi (family GH-72), has been predicted by combining results derived from threading methods, multiple sequence alignments and secondary-structure predictions. The 3D model has allowed the identification of several residues that are predicted to play a crucial role in structural integrity, substrate recognition and catalysis. In particular, the model of the catalytic domain can be useful for designing site-directed mutagenesis experiments and for developing inhibitors of Gas1p enzymatic activity.
Articolo in rivista - Articolo scientifico
protein structure prediction; computational methods; TIM barrel; glycosidase
English
237
248
Papaleo, E., Fantucci, P., Vai, M., & DE GIOIA, L. (2006). Three-dimensional structure of the catalytic domain of the yeast beta-(1,3)-glucan transferase Gas1: a molecular modeling investigation. JOURNAL OF MOLECULAR MODELING, 12(2), 237-248 [10.1007/s00894-005-0025-7].
Papaleo, E; Fantucci, P; Vai, M; DE GIOIA, L
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/1798
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