A HPLC assay was developed to determine IDN 5390, a new paclitaxel analogue, in mouse plasma. The method involves solid-phase extraction from cyano cartridges (recovery >80%), HPLC separation on Symmetry C(18) (4.6 x 150 mm), on isocratic mobile phase of water-acetonitrile-acetic acid (49:50:1) and detection at 227 nm. Retention times of IDN 5390 and IDN 5517 (internal standard, I.S.) were 9.1 and 10.5 min, respectively. The assay was linear from 0.05 to 5 micro g/ml (r(2)>or=0.995), showed intra- and inter-day precision within 1.0 and 6.2%, and accuracy of 94.7-106.8%. LOQ was 0.050 micro g/ml. Using this method IDN 5390 pharmacokinetics was determined in mice.

Zaffaroni, M., Frapolli, R., Colombo, T., Fruscio, R., Bombardelli, E., Morazzoni, P., et al. (2002). High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma. JOURNAL OF CHROMATOGRAPHY. B, 780(1), 93-98.

High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma

FRUSCIO, ROBERT;
2002-11-15

Abstract

A HPLC assay was developed to determine IDN 5390, a new paclitaxel analogue, in mouse plasma. The method involves solid-phase extraction from cyano cartridges (recovery >80%), HPLC separation on Symmetry C(18) (4.6 x 150 mm), on isocratic mobile phase of water-acetonitrile-acetic acid (49:50:1) and detection at 227 nm. Retention times of IDN 5390 and IDN 5517 (internal standard, I.S.) were 9.1 and 10.5 min, respectively. The assay was linear from 0.05 to 5 micro g/ml (r(2)>or=0.995), showed intra- and inter-day precision within 1.0 and 6.2%, and accuracy of 94.7-106.8%. LOQ was 0.050 micro g/ml. Using this method IDN 5390 pharmacokinetics was determined in mice.
Articolo in rivista - Articolo scientifico
Scientifica
Sensitivity and Specificity; Spectrophotometry, Ultraviolet; Bridged Compounds; Taxoids; Female; Paclitaxel; Mice; Chromatography, High Pressure Liquid; Animals; Reproducibility of Results
English
Zaffaroni, M., Frapolli, R., Colombo, T., Fruscio, R., Bombardelli, E., Morazzoni, P., et al. (2002). High-performance liquid chromatographic assay for the determination of the novel C-Seco-taxane derivative (IDN 5390) in mouse plasma. JOURNAL OF CHROMATOGRAPHY. B, 780(1), 93-98.
Zaffaroni, M; Frapolli, R; Colombo, T; Fruscio, R; Bombardelli, E; Morazzoni, P; Riva, A; D'Incalci, M; Zucchetti, M
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/17053
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