The expression of connexin 43 (cx43) and cell-cell communication were studied in replicative senescence of cultured HEL-299 fibroblasts. A progressive decrease in fluorescent dye transfer was detected by a scrape-loading technique in aging fibroblasts. This change was accounted for by a marked decrease in the amount of cx43 in aging cells, as detected by western blot analysis (cell extracts) and indirect fluorescence (cells in culture). However, semiquantitative RT-PCR assays of cx43 mRNA did not reveal appreciable changes, which suggests several possible explanations for the mechanism(s) underlying the decrease of cx43 in aging cells. These findings support the idea that the reduced expression of cx43 might be a biomarker of cell senescence

Statuto, M., Bianchi, C., Perego, R., Del Monte, U. (2002). Drop of connexin 43 in replicative senescence of human fibroblasts HEL-299 as a possible biomarker of senescence. EXPERIMENTAL GERONTOLOGY, 37(8-9), 1113-1120 [10.1016/S0531-5565(02)00089-X].

Drop of connexin 43 in replicative senescence of human fibroblasts HEL-299 as a possible biomarker of senescence

BIANCHI, CRISTINA;PEREGO, ROBERTO;
2002

Abstract

The expression of connexin 43 (cx43) and cell-cell communication were studied in replicative senescence of cultured HEL-299 fibroblasts. A progressive decrease in fluorescent dye transfer was detected by a scrape-loading technique in aging fibroblasts. This change was accounted for by a marked decrease in the amount of cx43 in aging cells, as detected by western blot analysis (cell extracts) and indirect fluorescence (cells in culture). However, semiquantitative RT-PCR assays of cx43 mRNA did not reveal appreciable changes, which suggests several possible explanations for the mechanism(s) underlying the decrease of cx43 in aging cells. These findings support the idea that the reduced expression of cx43 might be a biomarker of cell senescence
Articolo in rivista - Articolo scientifico
intercellular communication; gap junction; connexin 43; fibroblasts (human); replicative senescence
English
ago-2002
2002
37
8-9
1113
1120
none
Statuto, M., Bianchi, C., Perego, R., Del Monte, U. (2002). Drop of connexin 43 in replicative senescence of human fibroblasts HEL-299 as a possible biomarker of senescence. EXPERIMENTAL GERONTOLOGY, 37(8-9), 1113-1120 [10.1016/S0531-5565(02)00089-X].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/16519
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