Alzheimer's disease is characterised by brain neuritic plaques composed of a 39-44 amino acid peptide (Abeta). Lipid peroxidation is an early event induced by these amyloid beta-peptides, leading to the formation of 4-hydroxy-2-nonenal (HNE), which is one of the major end products of this process. HNE has been reported to form adducts via a stable covalent binding to proteins through a Michael addition to amino acid residues with a nucleophilic side chain. The present study reports an investigation of the conditions for formation of Abeta-HNE (Abeta 1-28 and Abeta 1-42) adducts, and their characterisation by liquid chromatography/electrospray ionisation mass spectrometry (LC/ESI-MS). The results suggest that one or more HNE moieties are localised in the 6-16 region of these adducts, while Asp-1, Lys-16 and Lys-28 are not modified under the described reaction conditions
Magni, F., Galbusera, C., Tremolada, L., Ferrarese, C., & Kienle, M.D. (2002). Characterisation of adducts of the lipid peroxidation product 4-hydroxy-2-nonenal and amyloid beta-peptides by liquid chromatography/electrospray ionisation mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 16(15), 1485-1493 [10.1002/rcm.743].
Citazione: | Magni, F., Galbusera, C., Tremolada, L., Ferrarese, C., & Kienle, M.D. (2002). Characterisation of adducts of the lipid peroxidation product 4-hydroxy-2-nonenal and amyloid beta-peptides by liquid chromatography/electrospray ionisation mass spectrometry. RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 16(15), 1485-1493 [10.1002/rcm.743]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Titolo: | Characterisation of adducts of the lipid peroxidation product 4-hydroxy-2-nonenal and amyloid beta-peptides by liquid chromatography/electrospray ionisation mass spectrometry | |
Autori: | Magni, F; Galbusera, C; Tremolada, L; Ferrarese, C; Kienle, MD | |
Autori: | ||
Data di pubblicazione: | 2002 | |
Lingua: | English | |
Rivista: | RAPID COMMUNICATIONS IN MASS SPECTROMETRY | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/rcm.743 | |
Appare nelle tipologie: | 01 - Articolo su rivista |