Rhodococcus opacus R7 is a naphthalene-degrading microorganism which is also able to grow on o-xylene. This work describes the isolation and analysis of two new genomic regions in which genes involved in naphthalene (nar gene cluster) and salicylate (gen gene cluster) degradation are located. In the nar gene cluster we found: two genes encoding the large (narAa) and the small (narAb) components of the naphthalene dioxygenase, three genes (rub1, rub2, rub1bis) encoding three rubredoxins, an orf (orf7) associated to the complex encoding a protein of unknown function, two regulatory genes (narR1, narR2), a gene (narB) encoding the naphthalene dihydrodiol dehydrogenase and six orfs (orf1, orf2, orf3, orf4, orf5, orf6) encoding proteins of unknown function. In the gen gene cluster, we found the following genes: two genes encoding the salicylate CoA ligase and the salicylate CoA synthetase (genA and genB), respectively, a gene (genC) encoding a salicylate hydroxylase, a gene (genH) encoding a gentisate 1,2-dioxygenase, a gene (genI) encoding a 3-maleylpyruvate isomerase, and a gene (genL) encoding a protein of unknown function. The transcription of some genes of R. opacus R7 strain grown on different substrates was also investigated to evaluate the expression of the two gene clusters after cDNA preparations

DI GENNARO, P., Masi, G., Botti, S., De Ferra, F., Papacchini, M., Bestetti, G. (2010). Identification and characterization of genes involved in naphthalene degradation in Rhodococcus opacus R7. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(1), 297-308 [10.1007/s00253-010-2497-3].

Identification and characterization of genes involved in naphthalene degradation in Rhodococcus opacus R7

DI GENNARO, PATRIZIA;BESTETTI, GIUSEPPINA
2010

Abstract

Rhodococcus opacus R7 is a naphthalene-degrading microorganism which is also able to grow on o-xylene. This work describes the isolation and analysis of two new genomic regions in which genes involved in naphthalene (nar gene cluster) and salicylate (gen gene cluster) degradation are located. In the nar gene cluster we found: two genes encoding the large (narAa) and the small (narAb) components of the naphthalene dioxygenase, three genes (rub1, rub2, rub1bis) encoding three rubredoxins, an orf (orf7) associated to the complex encoding a protein of unknown function, two regulatory genes (narR1, narR2), a gene (narB) encoding the naphthalene dihydrodiol dehydrogenase and six orfs (orf1, orf2, orf3, orf4, orf5, orf6) encoding proteins of unknown function. In the gen gene cluster, we found the following genes: two genes encoding the salicylate CoA ligase and the salicylate CoA synthetase (genA and genB), respectively, a gene (genC) encoding a salicylate hydroxylase, a gene (genH) encoding a gentisate 1,2-dioxygenase, a gene (genI) encoding a 3-maleylpyruvate isomerase, and a gene (genL) encoding a protein of unknown function. The transcription of some genes of R. opacus R7 strain grown on different substrates was also investigated to evaluate the expression of the two gene clusters after cDNA preparations
Articolo in rivista - Articolo scientifico
Rhodococcus, Naphthalene degradation, Catabolic genes, RT-PCR
English
2010
87
1
297
308
none
DI GENNARO, P., Masi, G., Botti, S., De Ferra, F., Papacchini, M., Bestetti, G. (2010). Identification and characterization of genes involved in naphthalene degradation in Rhodococcus opacus R7. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 87(1), 297-308 [10.1007/s00253-010-2497-3].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/16412
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