Identification of sulfonylureas in serum is important in the diagnosis of hypoglycemic crisis of unknown origin. Methods based on HPLC with UV or fluorescence detection may give false positive results. Mass spectrometry may successfully avoid this problem. The described method allows the simultaneous identification and quantification of tolbutamide, chlorpropamide, glibenclamide, and glipizide in human serum using one of the tested sulfonylureas as the internal standard. Serum purification is carried out by solid-phase extraction with ENVI-C18 cartridges and samples are analyzed by liquid chromatography-electrospray mass spectrometry. For all drugs, the limit of detection and the limit of quantification are about 2 and 10 ng/ml, respectively.

Magni, F., Marazzini, L., Pereira, S., Monti, L., Kienle, M. (2000). Identification of sulfonylureas in serum by electrospray mass spectrometry. ANALYTICAL BIOCHEMISTRY, 282(1), 136-141 [10.1006/abio.2000.4597].

Identification of sulfonylureas in serum by electrospray mass spectrometry

MAGNI, FULVIO;KIENLE, MARZIA DONATELLA
2000

Abstract

Identification of sulfonylureas in serum is important in the diagnosis of hypoglycemic crisis of unknown origin. Methods based on HPLC with UV or fluorescence detection may give false positive results. Mass spectrometry may successfully avoid this problem. The described method allows the simultaneous identification and quantification of tolbutamide, chlorpropamide, glibenclamide, and glipizide in human serum using one of the tested sulfonylureas as the internal standard. Serum purification is carried out by solid-phase extraction with ENVI-C18 cartridges and samples are analyzed by liquid chromatography-electrospray mass spectrometry. For all drugs, the limit of detection and the limit of quantification are about 2 and 10 ng/ml, respectively.
Articolo in rivista - Articolo scientifico
Sulfonylurea Compounds; Ultraviolet Rays; Chromatography, Liquid; Glipizide; Glyburide; Models, Chemical; Mass Spectrometry; Chlorpropamide; Tolbutamide; Calibration; Humans
English
15-giu-2000
282
1
136
141
none
Magni, F., Marazzini, L., Pereira, S., Monti, L., Kienle, M. (2000). Identification of sulfonylureas in serum by electrospray mass spectrometry. ANALYTICAL BIOCHEMISTRY, 282(1), 136-141 [10.1006/abio.2000.4597].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/16237
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