Activation of the cdc2 protein kinase at different stages of the cell cycle is regulated by post-translational modifications and interactions with cyclins. We show that in vitro translated human cdc2 binds very poorly to A and B cyclins, unless it has been preincubated with a Xenopus egg extract. This results in the phosphorylation of cdc2 which allows binding to cyclins. The replacement of Thr161, a residue conserved and phosphorylated in other protein kinases, with valine inhibits cdc2 association with A and B cyclins. In addition, mutations in the amino-terminus of cdc2 and within the conserved 'PSTAIR' region strongly inhibit binding. The Thr161Val mutation causes a lethal phenotype in the fission yeast Schizosaccharomyces pombe, while replacement of Thr161 with glutamic acid, potentially mimicking phosphorylation, causes uncoordination of mitosis and multiple cytokinesis. These results suggest that a threonine phosphorylation/dephosphorylation cycle is involved in regulating cdc2 function
Ducommun, B., Brambilla, P., Félix, M., Franza, B., Karsenti, E., & Draetta, G. (1991). cdc2 phosphorylation is required for its interaction with cyclin. EMBO JOURNAL, 10(11), 3311-3319.
Citazione: | Ducommun, B., Brambilla, P., Félix, M., Franza, B., Karsenti, E., & Draetta, G. (1991). cdc2 phosphorylation is required for its interaction with cyclin. EMBO JOURNAL, 10(11), 3311-3319. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | Si | |
Titolo: | cdc2 phosphorylation is required for its interaction with cyclin | |
Autori: | Ducommun, B; Brambilla, P; Félix, M; Franza, B; Karsenti, E; Draetta, G | |
Autori: | BRAMBILLA, PAOLO (Secondo) | |
Data di pubblicazione: | 1991 | |
Lingua: | English | |
Rivista: | EMBO JOURNAL | |
Appare nelle tipologie: | 01 - Articolo su rivista |
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