Conditioned medium obtained from the adrenocortical LAF1 mouse tumor (Y-1) cell cultures was able to stimulate the proliferation and the differentiation of granulocyte-macrophage precursors in the normal murine bone marrow. Colony-stimulating factor (CSF) was spontaneously produced by Y-1 cells also in serum-free cultures. By two cycles of gel chromatography on Sephadex G-150 of concentrated conditioned medium two peaks of colony-stimulating activity were isolated that corresponded to apparent molecular radii of 100,000 and 29,000, respectively. At this step of purification, the two factors gave a similar dose-response curve, showed a remarkable resistance to the heat treatment and pH changes, and were not extracted by ether. Because Y-1 cells resulted in infection by retrovirus, they provide a useful model to investigate the relations between viral coded information and CSF production

Pessina, A., Neri, M., Muschiato, A., Brambilla, P., Marocchi, A., Mocarelli, P. (1986). Colony-stimulating factor produced by murine adrenocortical tumor cells. JOURNAL OF THE NATIONAL CANCER INSTITUTE, 76(6), 1095-1099 [10.1093/jnci/76.6.1095].

Colony-stimulating factor produced by murine adrenocortical tumor cells

BRAMBILLA, PAOLO;MOCARELLI, PAOLO
Ultimo
1986

Abstract

Conditioned medium obtained from the adrenocortical LAF1 mouse tumor (Y-1) cell cultures was able to stimulate the proliferation and the differentiation of granulocyte-macrophage precursors in the normal murine bone marrow. Colony-stimulating factor (CSF) was spontaneously produced by Y-1 cells also in serum-free cultures. By two cycles of gel chromatography on Sephadex G-150 of concentrated conditioned medium two peaks of colony-stimulating activity were isolated that corresponded to apparent molecular radii of 100,000 and 29,000, respectively. At this step of purification, the two factors gave a similar dose-response curve, showed a remarkable resistance to the heat treatment and pH changes, and were not extracted by ether. Because Y-1 cells resulted in infection by retrovirus, they provide a useful model to investigate the relations between viral coded information and CSF production
Articolo in rivista - Articolo scientifico
Adrenal Cortex Neoplasms; Animals; Cell Line; Chromatography, Gel; Colony-Stimulating Factors; Culture Media; Hydrogen-Ion Concentration; Mice; Molecular Weight
English
1095
1099
5
Pessina, A., Neri, M., Muschiato, A., Brambilla, P., Marocchi, A., Mocarelli, P. (1986). Colony-stimulating factor produced by murine adrenocortical tumor cells. JOURNAL OF THE NATIONAL CANCER INSTITUTE, 76(6), 1095-1099 [10.1093/jnci/76.6.1095].
Pessina, A; Neri, M; Muschiato, A; Brambilla, P; Marocchi, A; Mocarelli, P
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/162258
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