Regeneration of muscle fibers that are lost during pathological muscle degeneration or after injuries is sustained by the production of new myofibers. An important cell type involved in muscle regeneration is the satellite cell. Necdin is a protein expressed in satellite cell-derived myogenic precursors during perinatal growth. However, its function in myogenesis is not known. We compare transgenic mice that overexpress necdin in skeletal muscle with both wild-type and necdin null mice. After muscle injury the necdin null mice show a considerable defect in muscle healing, whereas mice that overexpress necdin show a substantial increase in myofiber regeneration. We also find that in muscle, necdin increases myogenin expression, accelerates differentiation, and counteracts myoblast apoptosis. Collectively, these data clarify the function and mechanism of necdin in skeletal muscle and show the importance of necdin in muscle regeneration.

Deponti, D., Francois, S., Baesso, S., Sciorati, C., Innocenzi, A., Broccoli, V., et al. (2007). Necdin mediates skeletal muscle regeneration by promoting myoblast survival and differentiation. THE JOURNAL OF CELL BIOLOGY, 179(2), 305-319 [10.1083/jcb.200701027].

Necdin mediates skeletal muscle regeneration by promoting myoblast survival and differentiation.

FRANCOIS, STEPHANIE ANNE;MENEVERI, RAFFAELLA;BRUNELLI, SILVIA
2007

Abstract

Regeneration of muscle fibers that are lost during pathological muscle degeneration or after injuries is sustained by the production of new myofibers. An important cell type involved in muscle regeneration is the satellite cell. Necdin is a protein expressed in satellite cell-derived myogenic precursors during perinatal growth. However, its function in myogenesis is not known. We compare transgenic mice that overexpress necdin in skeletal muscle with both wild-type and necdin null mice. After muscle injury the necdin null mice show a considerable defect in muscle healing, whereas mice that overexpress necdin show a substantial increase in myofiber regeneration. We also find that in muscle, necdin increases myogenin expression, accelerates differentiation, and counteracts myoblast apoptosis. Collectively, these data clarify the function and mechanism of necdin in skeletal muscle and show the importance of necdin in muscle regeneration.
Articolo in rivista - Articolo scientifico
necdin, msatellite cells, muscle regeneration, myogenin, apoptosis
English
22-ott-2007
179
2
305
319
open
Deponti, D., Francois, S., Baesso, S., Sciorati, C., Innocenzi, A., Broccoli, V., et al. (2007). Necdin mediates skeletal muscle regeneration by promoting myoblast survival and differentiation. THE JOURNAL OF CELL BIOLOGY, 179(2), 305-319 [10.1083/jcb.200701027].
File in questo prodotto:
File Dimensione Formato  
DepontiJ Cell Biol2007Necdin mediates skeletal muscle regeneration.pdf

accesso aperto

Tipologia di allegato: Other attachments
Dimensione 3.76 MB
Formato Adobe PDF
3.76 MB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/1602
Citazioni
  • Scopus 46
  • ???jsp.display-item.citation.isi??? 47
Social impact