Using an immunocytochemical assay to monitor synaptic vesicle exocytosis/endocytosis independently of neurotransmitter release, we have investigated some aspects of vesicle recycling in hippocampal neurons at different developmental stages. A calcium- and depolarization-dependent exocytotic/endocytotic recycling of synaptic vesicles was found to take place in neurons already before the formation of synaptic contacts. The analysis of synaptic vesicle recycling at different calcium concentrations revealed the presence of two release components: the first one activated by low calcium concentrations and sustaining vesicle recycling before synaptogenesis, and a second one activated by high calcium concentrations, which is specifically turned on after the establishment of synaptic contacts. These data suggest that formation of synapses correlates with the activation of a putative low-affinity calcium sensor, which allows synaptic vesicle exocytosis to be triggered and turned off over extremely short time scales, in response to large increases in the level of intracellular calcium

Coco, S., Verderio, C., De Camilli, P., Matteoli, M. (1998). Calcium dependence of synaptic vesicle recycling before and after synaptogenesis. JOURNAL OF NEUROCHEMISTRY, 71(5), 1987-1992.

Calcium dependence of synaptic vesicle recycling before and after synaptogenesis

COCO, SILVIA
Primo
;
1998

Abstract

Using an immunocytochemical assay to monitor synaptic vesicle exocytosis/endocytosis independently of neurotransmitter release, we have investigated some aspects of vesicle recycling in hippocampal neurons at different developmental stages. A calcium- and depolarization-dependent exocytotic/endocytotic recycling of synaptic vesicles was found to take place in neurons already before the formation of synaptic contacts. The analysis of synaptic vesicle recycling at different calcium concentrations revealed the presence of two release components: the first one activated by low calcium concentrations and sustaining vesicle recycling before synaptogenesis, and a second one activated by high calcium concentrations, which is specifically turned on after the establishment of synaptic contacts. These data suggest that formation of synapses correlates with the activation of a putative low-affinity calcium sensor, which allows synaptic vesicle exocytosis to be triggered and turned off over extremely short time scales, in response to large increases in the level of intracellular calcium
Articolo in rivista - Articolo scientifico
Animals; Calcium; Cell Aging; Cells, Cultured; Exocytosis; Extracellular Space; Hippocampus; Neurons; Osmolar Concentration; Rats; Synapses; Synaptic Transmission; Synaptic Vesicles; Time Factors
English
1987
1992
6
Coco, S., Verderio, C., De Camilli, P., Matteoli, M. (1998). Calcium dependence of synaptic vesicle recycling before and after synaptogenesis. JOURNAL OF NEUROCHEMISTRY, 71(5), 1987-1992.
Coco, S; Verderio, C; De Camilli, P; Matteoli, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/158888
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