RalGPS2 is a guanine nucleotide exchange factor for RalA GTPase characterized by a C-terminal Pleckstrin Homology (PH) domain; this GEF is endogenously expressed in PC12 cells and in rat brain but its role in PC12 cells and in cell differentiation is actually unknown. Here we have shown that transient expression of RalGPS2-PH-PxxP domain in PC12 and PC12-TrkA cells induces high level of neurite outgrowth; this differentiation is comparable with that of PC12 cells treated with RalGPS2 siRNA. Stable PC12 cell lines expressing the PH-PxxP domain of RalGPS2 have been generated; in these cell lines the PH-PxxP domain acts as a dominant negative for RalA activation, promotes cells differentiation and redirects NGF signals towards MAPKs. Furthermore it has been also demonstrated that the PH-PxxP domain of RalGPS2 inducesmicrospikes formation a typical feature of cells in which the Cdc42 GTPase is constitutively activated
Ceriani, M., Amigoni, L., Scandiuzzi, C., Berruti, G., & Martegani, E. (2010). The PH-PxxP domain of RalGPS2 promotes PC12 cells differentiation acting as a dominant negative for RalA GTPase activation. NEUROSCIENCE RESEARCH, 66(3), 290-298.
Citazione: | Ceriani, M., Amigoni, L., Scandiuzzi, C., Berruti, G., & Martegani, E. (2010). The PH-PxxP domain of RalGPS2 promotes PC12 cells differentiation acting as a dominant negative for RalA GTPase activation. NEUROSCIENCE RESEARCH, 66(3), 290-298. |
Tipo: | Articolo in rivista - Articolo scientifico |
Carattere della pubblicazione: | Scientifica |
Titolo: | The PH-PxxP domain of RalGPS2 promotes PC12 cells differentiation acting as a dominant negative for RalA GTPase activation |
Autori: | Ceriani, M; Amigoni, L; Scandiuzzi, C; Berruti, G; Martegani, E |
Autori: | |
Data di pubblicazione: | mar-2010 |
Lingua: | English |
Rivista: | NEUROSCIENCE RESEARCH |
Appare nelle tipologie: | 01 - Articolo su rivista |