In this study, we evaluated the anti-amyloid effect of functionalized nanoliposomes (mApoE-PA-LIP) in a mouse model of Alzheimer's disease with use of positron emission tomography and β-amyloid (Aβ)–targeted tracer [11C]Pittsburgh compound B ([11C]PIB). APP23 mice were injected with mApoE-PA-LIP or saline (3 times per week for 3 weeks) and [11C]PIB imaging was performed at baseline, after the treatment and after 3 months follow-up period, accompanied by Aβ immunohistochemistry and ELISA. After the treatment, [11C]PIB binding ratios between mApoE-PA-LIP and saline groups were equivalent in all analyzed brain regions; however, in the saline group, binding ratios increased from the baseline, whereas no increase was detected in the mApoE-PA-LIP group. During the additional follow-up, [11C]PIB binding increased significantly from baseline in both groups, and binding ratios correlated with the immunohistochemically defined Aβ load. This study further supports the use of [11C]PIB positron emission tomography imaging as a biomarker of Aβ deposition in APP23 mice and highlights the benefits of noninvasive follow-up, that is, using baseline data for animal stratification and normalization of treatment effects to baseline values, for future anti-amyloid treatment studies.
Snellmana, A., Rokka, J., Lopez Picona, F., Helin, S., Re, F., Löyttyniemi, E., et al. (2017). Applicability of [11C]PIB micro-PET imaging for in vivo follow-up of anti-amyloid treatment effects in APP23 mouse model. NEUROBIOLOGY OF AGING, 57, 84-94 [10.1016/j.neurobiolaging.2017.05.008].
|Citazione:||Snellmana, A., Rokka, J., Lopez Picona, F., Helin, S., Re, F., Löyttyniemi, E., et al. (2017). Applicability of [11C]PIB micro-PET imaging for in vivo follow-up of anti-amyloid treatment effects in APP23 mouse model. NEUROBIOLOGY OF AGING, 57, 84-94 [10.1016/j.neurobiolaging.2017.05.008].|
|Tipo:||Articolo in rivista - Articolo scientifico|
|Carattere della pubblicazione:||Scientifica|
|Presenza di un coautore afferente ad Istituzioni straniere:||Si|
|Titolo:||Applicability of [11C]PIB micro-PET imaging for in vivo follow-up of anti-amyloid treatment effects in APP23 mouse model|
|Autori:||Snellmana, A; Rokka, J; Lopez Picona, F; Helin, S; Re, F; Löyttyniemi, E; Pihlajaa, R; Forloni, G; Salmona, M; Masserini, M; Olof, S; Juha O., R; Merja Haaparanta, S|
MASSERINI, MASSIMO ERNESTO (Ultimo)
|Data di pubblicazione:||2017|
|Rivista:||NEUROBIOLOGY OF AGING|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.neurobiolaging.2017.05.008|
|Appare nelle tipologie:||01 - Articolo su rivista|