Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infections in infants and young children. RSV is characterised by high variability, especially in the G glycoprotein, which may play a significant role in RSV pathogenicity by allowing immune evasion. To reconstruct the origin and phylodynamic history of RSV, we evaluated the genetic diversity and evolutionary dynamics of RSV A and RSV B isolated from children under 3 years old infected in Italy from 2006 to 2012. Phylogenetic analysis revealed that most of the RSV A sequences clustered with the NA1 genotype, and RSV B sequences were included in the Buenos Aires genotype. The mean evolutionary rates for RSV A and RSV B were estimated to be 2.1×10-3 substitutions (subs)/site/year and 3.03×10-3 subs/site/year, respectively. The time of most recent common ancestor for the tree root went back to the 1940s (95% highest posterior density-HPD: 1927-1951) for RSV A and the 1950s (95%HPD: 1951-1960) for RSV B. The RSV A Bayesian skyline plot (BSP) showed a decrease in transmission events ending in about 2005, when a sharp growth restored the original viral population size. RSV B BSP showed a similar trend. Site-specific selection analysis identified 10 codons under positive selection in RSV A sequences and only one site in RSV B sequences. Although RSV remains difficult to control due to its antigenic diversity, it is important to monitor changes in its coding sequences, to permit the identification of future epidemic strains and to implement vaccine and therapy strategies. © 2014 Elsevier B.V

Martinelli, M., Frati, E., Zappa, A., Ebranati, E., Bianchi, S., Pariani, E., et al. (2014). Phylogeny and population dynamics of respiratory syncytial virus (Rsv) A and B. VIRUS RESEARCH, 189, 293-302 [10.1016/j.virusres.2014.06.006].

Phylogeny and population dynamics of respiratory syncytial virus (Rsv) A and B

MARTINELLI, MARIANNA
Primo
;
2014

Abstract

Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infections in infants and young children. RSV is characterised by high variability, especially in the G glycoprotein, which may play a significant role in RSV pathogenicity by allowing immune evasion. To reconstruct the origin and phylodynamic history of RSV, we evaluated the genetic diversity and evolutionary dynamics of RSV A and RSV B isolated from children under 3 years old infected in Italy from 2006 to 2012. Phylogenetic analysis revealed that most of the RSV A sequences clustered with the NA1 genotype, and RSV B sequences were included in the Buenos Aires genotype. The mean evolutionary rates for RSV A and RSV B were estimated to be 2.1×10-3 substitutions (subs)/site/year and 3.03×10-3 subs/site/year, respectively. The time of most recent common ancestor for the tree root went back to the 1940s (95% highest posterior density-HPD: 1927-1951) for RSV A and the 1950s (95%HPD: 1951-1960) for RSV B. The RSV A Bayesian skyline plot (BSP) showed a decrease in transmission events ending in about 2005, when a sharp growth restored the original viral population size. RSV B BSP showed a similar trend. Site-specific selection analysis identified 10 codons under positive selection in RSV A sequences and only one site in RSV B sequences. Although RSV remains difficult to control due to its antigenic diversity, it is important to monitor changes in its coding sequences, to permit the identification of future epidemic strains and to implement vaccine and therapy strategies. © 2014 Elsevier B.V
Articolo in rivista - Articolo scientifico
Evolutionary rate; G protein; Phylodynamic analysis; Phylogenetic analysis; Respiratory syncytial virus; Selective pressure; Child, Preschool; Evolution, Molecular; Female; Humans; Infant; Italy; Male; Molecular Epidemiology; Molecular Sequence Data; RNA, Viral; Respiratory Syncytial Virus Infections; Respiratory Syncytial Virus, Human; Sequence Analysis, DNA; Genetic Variation; Phylogeny; Cancer Research; Virology; Infectious Diseases
English
2014
189
293
302
none
Martinelli, M., Frati, E., Zappa, A., Ebranati, E., Bianchi, S., Pariani, E., et al. (2014). Phylogeny and population dynamics of respiratory syncytial virus (Rsv) A and B. VIRUS RESEARCH, 189, 293-302 [10.1016/j.virusres.2014.06.006].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/156111
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