Background: Polyomavirus (PyV) infection is common, ranging from 60% to 100% depending on the virus. The urinary excretion rates of JC virus (JCV) and BK virus (BKV) have been extensively studied, but less is known about the more recently discovered PyVs. Objectives: To investigate the urinary excretion of Merkel cell PyV (MCPyV), which is associated with Merkel cell carcinoma (MCC), in kidney transplant recipients and healthy subjects, as well as those of lymphotropic polyomavirus (LPV), which was isolated from the B-cells of African green monkeys but has also been found in human blood, JCV and BKV. Study design: Urine samples were collected from 62 adult (ATP) and 46 pediatric (PTP) kidney transplant recipients and from 67 adult (AHC) and 40 pediatric (PHC) healthy controls. DNA was isolated and analyzed using real-time PCR (Q-PCR) to determine the viral loads of MCPyV, LPV, JCV and BKV. Results: MCPyV DNA was more frequently detected (p<. 0.05) in the PTP (36.9%) and PHC (30.0%) groups compared to JCV (8.7% in PTP, 12.5% in PHC), BKV (6.5% in PTP, 2.5% in PHC), and LPV (2.2% in PTP, 5% in PHC) and in the AHC (47.8%) group compared to BKV (13.4%) and LPV (0%). Conclusions: Based on the results, it could be concluded that: (a) Despite the rarity of MCC, MCPyV is a common infection; (b) MCPyV demonstrates an excretion pattern similar to those of JCV and BKV, persisting in the kidney and infecting skin cells upon reactivation, with subsequent integration and transformation

Signorini, L., Belingheri, M., Ambrogi, F., Pagani, E., Binda, S., Ticozzi, R., et al. (2014). High frequency of Merkel cell polyomavirus DNA in the urine of kidney transplant recipients and healthy controls. JOURNAL OF CLINICAL VIROLOGY, 61(4), 565-570 [10.1016/j.jcv.2014.10.012].

High frequency of Merkel cell polyomavirus DNA in the urine of kidney transplant recipients and healthy controls

SIGNORINI, LUCIA
Primo
;
2014

Abstract

Background: Polyomavirus (PyV) infection is common, ranging from 60% to 100% depending on the virus. The urinary excretion rates of JC virus (JCV) and BK virus (BKV) have been extensively studied, but less is known about the more recently discovered PyVs. Objectives: To investigate the urinary excretion of Merkel cell PyV (MCPyV), which is associated with Merkel cell carcinoma (MCC), in kidney transplant recipients and healthy subjects, as well as those of lymphotropic polyomavirus (LPV), which was isolated from the B-cells of African green monkeys but has also been found in human blood, JCV and BKV. Study design: Urine samples were collected from 62 adult (ATP) and 46 pediatric (PTP) kidney transplant recipients and from 67 adult (AHC) and 40 pediatric (PHC) healthy controls. DNA was isolated and analyzed using real-time PCR (Q-PCR) to determine the viral loads of MCPyV, LPV, JCV and BKV. Results: MCPyV DNA was more frequently detected (p<. 0.05) in the PTP (36.9%) and PHC (30.0%) groups compared to JCV (8.7% in PTP, 12.5% in PHC), BKV (6.5% in PTP, 2.5% in PHC), and LPV (2.2% in PTP, 5% in PHC) and in the AHC (47.8%) group compared to BKV (13.4%) and LPV (0%). Conclusions: Based on the results, it could be concluded that: (a) Despite the rarity of MCC, MCPyV is a common infection; (b) MCPyV demonstrates an excretion pattern similar to those of JCV and BKV, persisting in the kidney and infecting skin cells upon reactivation, with subsequent integration and transformation
Articolo in rivista - Articolo scientifico
Kidney transplant recipients; Merkel cell polyomavirus; Polyomaviruses; Urinary excretion; Adolescent; Adult; Aged; Aged, 80 and over; Child; Child, Preschool; DNA, Viral; Female; Healthy Volunteers; Humans; Infant; Male; Middle Aged; Polyomavirus; Real-Time Polymerase Chain Reaction; Urine; Viral Load; Young Adult; Kidney Transplantation; Transplant Recipients; Infectious Diseases; Virology
English
2014
61
4
565
570
none
Signorini, L., Belingheri, M., Ambrogi, F., Pagani, E., Binda, S., Ticozzi, R., et al. (2014). High frequency of Merkel cell polyomavirus DNA in the urine of kidney transplant recipients and healthy controls. JOURNAL OF CLINICAL VIROLOGY, 61(4), 565-570 [10.1016/j.jcv.2014.10.012].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/154971
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