Here, we describe the use of complementary techniques applicable to different types of samples to analyze chromosomal alterations in urothelial carcinoma. By a conventional chromosome analysis on fresh biopsies, it is possible to delineate the status of ploidy and rough chromosomal aberrations. The multi-target fluorescence in situ hybridization (FISH) UroVysion test, for the rapid detection of chromosomal aneusomy of chromosomes 3, 7, and 17 and/or deletion of 9p21 locus, is applicable to urine specimens as well as to formalin-fixed paraffin-embedded (FFPE) specimens and fresh biopsies. Finally, array comparative genomic hybridization (array-CGH) gives the possibility of analyzing the DNA in a single experiment from a biopsy of the tumor but also from FFPE specimens; this technique is able to detect alterations at the genome level not excluding any chromosome.
Conconi, D., & Bentivegna, A. (2018). Analysis of chromosomal alterations in urothelial carcinoma. In Methods in Molecular Biology: Urothelial Carcinoma (pp. 3-17). Humana Press Inc. [10.1007/978-1-4939-7234-0_1].
Citazione: | Conconi, D., & Bentivegna, A. (2018). Analysis of chromosomal alterations in urothelial carcinoma. In Methods in Molecular Biology: Urothelial Carcinoma (pp. 3-17). Humana Press Inc. [10.1007/978-1-4939-7234-0_1]. | |
Titolo: | Analysis of chromosomal alterations in urothelial carcinoma | |
Autori: | Conconi, D; Bentivegna, A | |
Autori: | CONCONI, DONATELLA (Primo) BENTIVEGNA, ANGELA (Ultimo) (Corresponding) | |
Presenza di un coautore afferente ad Istituzioni straniere: | No | |
Tipo: | Capitolo o saggio | |
Carattere della pubblicazione: | Scientifica | |
Data di pubblicazione: | 2018 | |
Lingua: | English | |
Titolo del libro: | Methods in Molecular Biology: Urothelial Carcinoma | |
ISBN: | 978-1-4939-7234-0 | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1007/978-1-4939-7234-0_1 | |
Appare nelle tipologie: | 03 - Contributo in libro |