Ligand-bound receptors of the Transforming Growth Factor-β (TGF-β) family promote the formation of complexes between Smad proteins that subsequently accumulate in the nucleus and interact there with other transcriptional regulators, leading to modulation of target gene expression. We identified a novel nuclear protein, Smicl, which binds to Smad proteins. Smicl and Smads cooperate and enhance TGF-β mediated activation of a Smad-responsive reporter gene. A domain with five CCCH-type zinc fingers in Smicl is structurally and functionally, at least in vitro, similar to a domain in CPSF-30, the 30 kDa subunit of Cleavage and Polyadenylation Specificity Factor (CPSF). Like CPSF-30, Smicl can associate with some other CPSF subunits characterized previously. Its effect on the induction of a reporter gene for TGF-β requires the cleavage/polyadenylation signal downstream of the coding sequence of that gene. Thus, Smicl is a novel protein that displays CPSF-30-like activities, interacts in the nucleus with activated Smads, and potentiates in TGF-β stimulated cells Smad-dependent transcriptional responses, possibly in conjunction with the activity of CPSF complexes. © Blackwell Publishing Limited
Collart, C., Remacle, J., Barabino, S., van Grunsven, L., Nelles, L., Schellens, A., et al. (2005). Smicl is a novel Smad interacting protein and cleavage and polyadenylation specificity factor associated protein. GENES TO CELLS, 10(9), 897-906 [10.1111/j.1365-2443.2005.00887.x].
Citazione: | Collart, C., Remacle, J., Barabino, S., van Grunsven, L., Nelles, L., Schellens, A., et al. (2005). Smicl is a novel Smad interacting protein and cleavage and polyadenylation specificity factor associated protein. GENES TO CELLS, 10(9), 897-906 [10.1111/j.1365-2443.2005.00887.x]. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | Si | |
Titolo: | Smicl is a novel Smad interacting protein and cleavage and polyadenylation specificity factor associated protein | |
Autori: | Collart, C; Remacle, J; Barabino, S; van Grunsven, L; Nelles, L; Schellens, A; Van de Putte, T; Pype, S; Huylebroeck, D; Verschueren, K | |
Autori: | ||
Data di pubblicazione: | 2005 | |
Lingua: | English | |
Rivista: | GENES TO CELLS | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1111/j.1365-2443.2005.00887.x | |
Appare nelle tipologie: | 01 - Articolo su rivista |