The Ras-specific guanine nucleotide exchange region of hSos1 consists of two consecutive domains: the catalytic core (residues 742-1024) contains all residues binding to Ras, including the catalytic hairpin, and an upstream REM domain (residues 553-741), so called because it contains an evolutionary conserved Ras Exchange Motif (REM). We functionally define the boundaries of the REM domain through a combination of in vivo and in vitro assays. We show that an intra-REM domain interaction, mediated by phenylalanine 577, is required to allow interaction of the REM domain with the catalytic core, constraining it in the active conformation.

Sacco, E., Metalli, D., Busti, S., Fantinato, S., D'Urzo, A., Mapelli, V., et al. (2006). Catalytic competence of the Ras-GEF domain of hSos1 requires intra-REM domain interactions mediated by phenylalanine 577. FEBS LETTERS, 580(27), 6322-6328 [10.1016/j.febslet.2006.10.040].

Catalytic competence of the Ras-GEF domain of hSos1 requires intra-REM domain interactions mediated by phenylalanine 577

SACCO, ELENA;METALLI, DAVID;BUSTI, STEFANO;D'URZO, ANNALISA;Mapelli, V;ALBERGHINA, LILIA;VANONI, MARCO ERCOLE
2006

Abstract

The Ras-specific guanine nucleotide exchange region of hSos1 consists of two consecutive domains: the catalytic core (residues 742-1024) contains all residues binding to Ras, including the catalytic hairpin, and an upstream REM domain (residues 553-741), so called because it contains an evolutionary conserved Ras Exchange Motif (REM). We functionally define the boundaries of the REM domain through a combination of in vivo and in vitro assays. We show that an intra-REM domain interaction, mediated by phenylalanine 577, is required to allow interaction of the REM domain with the catalytic core, constraining it in the active conformation.
Articolo in rivista - Articolo scientifico
Biacore; Catalytic region; Guanine nucleotide exchange factor; Intramolecular interaction; Mutagenesis;
English
2006
580
27
6322
6328
none
Sacco, E., Metalli, D., Busti, S., Fantinato, S., D'Urzo, A., Mapelli, V., et al. (2006). Catalytic competence of the Ras-GEF domain of hSos1 requires intra-REM domain interactions mediated by phenylalanine 577. FEBS LETTERS, 580(27), 6322-6328 [10.1016/j.febslet.2006.10.040].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/15392
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