The embryotoxic potential of chlorpyrifos (CPF) and malathion (MTN), two organophosphorus insecticides (OPs), was evaluated by modified Frog Embryo Teratogenesis Assay-Xenopus (FETAX). CPF and MTN were not embryolethal even at the highest concentration tested (6000 microg/l), but both exhibited a powerful teratogenicity. The probit analysis of malformed larva percentages showed a TC(50) of 161.54mug/l for CPF, and a TC(50) of 2394.01 microg/l for MTN. Therefore, CPF teratogenicity was about 15 times higher than MTN. Larvae of both exposed groups were mainly affected by ventral and/or lateral tail flexure coupled with abnormal gut coiling. Histopathological diagnosis displayed abnormal myotomes and myocytes with marked hypertrophies localized at the cell extremity, probably due to a break away of myofibril extremities at the intersomitic junction level. We speculate that this muscular damage was related to inhibition of acetylcholinesterase that showed a clear concentration-response in CPF and MTN exposed larvae. The teratogenic effects of these anti-cholinesterase compounds on Xenopus laevis myogenesis suggest a possible role played by OPs on induction of congenital muscular dystrophy.

Bonfanti, P., Colombo, A., Orsi, F., Nizzetto, I., Andrioletti, M., Bacchetta, R., et al. (2004). Comparative teratogenicity of Chlorpyrifos and Malathion on Xenopus laevis development. AQUATIC TOXICOLOGY, 70(3), 189-200 [10.1016/j.aquatox.2004.09.007].

Comparative teratogenicity of Chlorpyrifos and Malathion on Xenopus laevis development

BONFANTI, PATRIZIA;COLOMBO, ANITA EMILIA;MANTECCA, PARIDE;
2004

Abstract

The embryotoxic potential of chlorpyrifos (CPF) and malathion (MTN), two organophosphorus insecticides (OPs), was evaluated by modified Frog Embryo Teratogenesis Assay-Xenopus (FETAX). CPF and MTN were not embryolethal even at the highest concentration tested (6000 microg/l), but both exhibited a powerful teratogenicity. The probit analysis of malformed larva percentages showed a TC(50) of 161.54mug/l for CPF, and a TC(50) of 2394.01 microg/l for MTN. Therefore, CPF teratogenicity was about 15 times higher than MTN. Larvae of both exposed groups were mainly affected by ventral and/or lateral tail flexure coupled with abnormal gut coiling. Histopathological diagnosis displayed abnormal myotomes and myocytes with marked hypertrophies localized at the cell extremity, probably due to a break away of myofibril extremities at the intersomitic junction level. We speculate that this muscular damage was related to inhibition of acetylcholinesterase that showed a clear concentration-response in CPF and MTN exposed larvae. The teratogenic effects of these anti-cholinesterase compounds on Xenopus laevis myogenesis suggest a possible role played by OPs on induction of congenital muscular dystrophy.
Articolo in rivista - Articolo scientifico
Cholinesterase Inhibitors; Chlorpyrifos; Tail; Biological Assay; Malathion; Abnormalities, Drug-Induced; Muscles; Animals; Histological Techniques; Xenopus laevis
English
189
200
12
Bonfanti, P., Colombo, A., Orsi, F., Nizzetto, I., Andrioletti, M., Bacchetta, R., et al. (2004). Comparative teratogenicity of Chlorpyrifos and Malathion on Xenopus laevis development. AQUATIC TOXICOLOGY, 70(3), 189-200 [10.1016/j.aquatox.2004.09.007].
Bonfanti, P; Colombo, A; Orsi, F; Nizzetto, I; Andrioletti, M; Bacchetta, R; Mantecca, P; Fascio, U; Vailati, G; Vismara, C
File in questo prodotto:
File Dimensione Formato  
Bonfanti et al. 2004 (Aquatic Toxicology).pdf

accesso aperto

Dimensione 633.38 kB
Formato Adobe PDF
633.38 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/15347
Citazioni
  • Scopus 66
  • ???jsp.display-item.citation.isi??? 64
Social impact