Gene-targeted mice were used to evaluate the role of the gamma isoform of phosphoinositide 3-kinase (PI3Kgamma) in dendritic cell (DC) migration and induction of specific T-cell-mediated immune responses. DC obtained from PI3Kgamma-/- mice showed a reduced ability to respond to chemokines in vitro and ex vivo and to travel to draining lymph nodes under inflammatory conditions. PI3Kgamma-/- mice had a selective defect in the number of skin Langerhans cells and in lymph node CD8alpha- DC. Furthermore, PI3Kgamma-/- mice showed a defective capacity to mount contact hypersensitivity and delayed-type hypersensitivity reactions. This defect was directly related to the reduced ability of antigen-loaded DC to migrate from the periphery to draining lymph nodes. Thus, PI3Kgamma plays a nonredundant role in DC trafficking and in the activation of specific immunity. Therefore, PI3Kgamma may be considered a new target to control exaggerated immune reactions.

Del Prete, A., Vermi, W., Dander, E., Otero, K., Barberis, L., Luini, W., et al. (2004). Defective dendritic cell migration and activation of adaptive immunity in PI3Kγ-deficient mice. EMBO JOURNAL, 23(17), 3505-3515 [10.1038/sj.emboj.7600361].

Defective dendritic cell migration and activation of adaptive immunity in PI3Kγ-deficient mice

DANDER, ERICA;
2004

Abstract

Gene-targeted mice were used to evaluate the role of the gamma isoform of phosphoinositide 3-kinase (PI3Kgamma) in dendritic cell (DC) migration and induction of specific T-cell-mediated immune responses. DC obtained from PI3Kgamma-/- mice showed a reduced ability to respond to chemokines in vitro and ex vivo and to travel to draining lymph nodes under inflammatory conditions. PI3Kgamma-/- mice had a selective defect in the number of skin Langerhans cells and in lymph node CD8alpha- DC. Furthermore, PI3Kgamma-/- mice showed a defective capacity to mount contact hypersensitivity and delayed-type hypersensitivity reactions. This defect was directly related to the reduced ability of antigen-loaded DC to migrate from the periphery to draining lymph nodes. Thus, PI3Kgamma plays a nonredundant role in DC trafficking and in the activation of specific immunity. Therefore, PI3Kgamma may be considered a new target to control exaggerated immune reactions.
Articolo in rivista - Articolo scientifico
Chemokines, cell migration, Knockout mice; Animals; 1-Phosphatidylinositol 3-Kinase; Dendric cells
English
2004
23
17
3505
3515
none
Del Prete, A., Vermi, W., Dander, E., Otero, K., Barberis, L., Luini, W., et al. (2004). Defective dendritic cell migration and activation of adaptive immunity in PI3Kγ-deficient mice. EMBO JOURNAL, 23(17), 3505-3515 [10.1038/sj.emboj.7600361].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/15262
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