TD, produced by tire wear, is a significant constituent of PM(10) in urban areas where traffic related emissions are predominant. TD contains a lot of chemicals which can affect human respiratory system and it has received little attention until now, even the toxicity of PM has been extensively documented. A549 cells, a human alveolar lung cells, were exposed for 24, 48, 72 h to 10, 50, 60, 75 microg/ml of TD organic extract. MTT and Trypan Blue assays were used to evaluate cytotoxicity and Comet Assay to evidence DNA damage. TD extracts induced a dose-dependent increase in cell mortality and DNA damage. A significant toxicity was observed when cells were exposed to 60 microg/ml for 72 h. Moreover cell morphology observed at ultra structural level, was severely affected at the highest dose.

Gualtieri, M., Rigamonti, L., Galeotti, V., Camatini, M. (2005). Toxicity of tire debris extracts on human lung cell line A549. TOXICOLOGY IN VITRO, 19(7), 1001-1008 [10.1016/j.tiv.2005.06.038].

Toxicity of tire debris extracts on human lung cell line A549

GUALTIERI, MAURIZIO;RIGAMONTI, LAURA;CAMATINI, MARINA CARLA
2005

Abstract

TD, produced by tire wear, is a significant constituent of PM(10) in urban areas where traffic related emissions are predominant. TD contains a lot of chemicals which can affect human respiratory system and it has received little attention until now, even the toxicity of PM has been extensively documented. A549 cells, a human alveolar lung cells, were exposed for 24, 48, 72 h to 10, 50, 60, 75 microg/ml of TD organic extract. MTT and Trypan Blue assays were used to evaluate cytotoxicity and Comet Assay to evidence DNA damage. TD extracts induced a dose-dependent increase in cell mortality and DNA damage. A significant toxicity was observed when cells were exposed to 60 microg/ml for 72 h. Moreover cell morphology observed at ultra structural level, was severely affected at the highest dose.
Articolo in rivista - Articolo scientifico
Cell Survival; Time Factors; Cell Line; DNA Damage; Particle Size; Environmental Pollutants; Dose-Response Relationship, Drug; Pulmonary Alveoli; Comet Assay; Rubber; Cell Cycle; Humans
English
2005
19
7
1001
1008
none
Gualtieri, M., Rigamonti, L., Galeotti, V., Camatini, M. (2005). Toxicity of tire debris extracts on human lung cell line A549. TOXICOLOGY IN VITRO, 19(7), 1001-1008 [10.1016/j.tiv.2005.06.038].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/15238
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