Aim: Based on the evidence that microvesicles (MVs) shed from Bone -Marrow Mesenchymal Stem Cells (BMMSCs) can play an immunomodulatory role, protecting the injured tissue, we aimed at assessing the possible anti-inflammatory effects of BM-MSCs-deriving MVs in microglia cultures exposed to hAbeta 1-42. Methods: MV swere isolated by ultracentrifugation from mouse BM-MSCs and then characterized by FACS analysis. MVs were then incubated with primary microglia in the presence of human 1-42 Abeta peptide. The expression of different inflammatory or anti-inflammatory markerswas investigated by ELISA, FACS, RTPCR and immunocytochemistry. Results:Microglial cells assume an amoeboid phenotype, changing its morphology and releasing antiinflammatory cytokines, such as IL10, without significantly affecting the release of the pro-inflammatory cytokines TNFa and IL6. Also, MVs lead to the decrease in the expression ofmarkers like MHC II, which is associated with a pro-inflammatory phenotype. Conclusions: BM-MSCMVs down-regulate the inflammatory processes induced by exposureof microglia to h 1-42 Abeta peptide in vitro. The involved molecular mechanisms are under investigation.

Elia, C., Mazzitelli, S., Filipello, F., Marchetti, S., Rasile, M., Tamborini, M., et al. (2015). Microvesicles from Mesenchymal Stem Cells Induce an Anti-Inflammatory Phenotype of Microglia Exposed to Human Abeta 1-42. In Mechanisms, Clinical Strategies, and Promising Treatments of Neurodegenerative Diseases. 12th International Conference AD/PDTM Nice, France, 18-22 March 2015 (pp.806-806).

Microvesicles from Mesenchymal Stem Cells Induce an Anti-Inflammatory Phenotype of Microglia Exposed to Human Abeta 1-42

MARCHETTI, SARA;COCO, SILVIA
Penultimo
;
2015

Abstract

Aim: Based on the evidence that microvesicles (MVs) shed from Bone -Marrow Mesenchymal Stem Cells (BMMSCs) can play an immunomodulatory role, protecting the injured tissue, we aimed at assessing the possible anti-inflammatory effects of BM-MSCs-deriving MVs in microglia cultures exposed to hAbeta 1-42. Methods: MV swere isolated by ultracentrifugation from mouse BM-MSCs and then characterized by FACS analysis. MVs were then incubated with primary microglia in the presence of human 1-42 Abeta peptide. The expression of different inflammatory or anti-inflammatory markerswas investigated by ELISA, FACS, RTPCR and immunocytochemistry. Results:Microglial cells assume an amoeboid phenotype, changing its morphology and releasing antiinflammatory cytokines, such as IL10, without significantly affecting the release of the pro-inflammatory cytokines TNFa and IL6. Also, MVs lead to the decrease in the expression ofmarkers like MHC II, which is associated with a pro-inflammatory phenotype. Conclusions: BM-MSCMVs down-regulate the inflammatory processes induced by exposureof microglia to h 1-42 Abeta peptide in vitro. The involved molecular mechanisms are under investigation.
No
abstract + poster
microvesicles; Bone -Marrow Mesenchymal Stem Cells; Alzheimer's disease
English
Mechanisms, Clinical Strategies, and Promising Treatments of Neurodegenerative Diseases. 12th International Conference AD/PDTM
Elia, C., Mazzitelli, S., Filipello, F., Marchetti, S., Rasile, M., Tamborini, M., et al. (2015). Microvesicles from Mesenchymal Stem Cells Induce an Anti-Inflammatory Phenotype of Microglia Exposed to Human Abeta 1-42. In Mechanisms, Clinical Strategies, and Promising Treatments of Neurodegenerative Diseases. 12th International Conference AD/PDTM Nice, France, 18-22 March 2015 (pp.806-806).
Elia, C; Mazzitelli, S; Filipello, F; Marchetti, S; Rasile, M; Tamborini, M; Coco, S; Matteoli, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/151368
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