Cultured human fibroblasts were fed with two differently labelled sulphatide molecules [one labelled on C-3 of the sphingosine (Sph) moiety ([Sph-3H]sulphatide), the second on C-1 of stearic acid ([stearoyl-14C]sulphatide)], and the intracellular metabolic fate of radioactivity was monitored. Incorporated radioactivity was almost all recovered in the total lipid extract, regardless of the labelling position of the added sulphatide; however, large differences in the level of incorporation occurred among labelled glycosphingolipids. For example, sphingomyelin was present as the major radiolabelled lipid after [Sph-3H]sulphatide incubation, but was detectable only in trace amounts after [stearoyl-14C]sulphatide administration; in the latter case the radioactivity was located predominantly in glycerophospholipids. From this finding it can be inferred that the free long-chain base (sphingosine) that originates from lysosomal catabolism of sulphatide is mainly, and quite specifically, utilized for sphingomyelin biosynthesis, whereas the ceramide moiety is not; conversely the fatty acid release from ceramide is non-specifically re-utilized for phospholipid biosynthesis.
Trinchera, M., Wiesmann, U., Pitto, M., Acquotti, D., & Ghidoni, R. (1988). Different metabolic recycling of the lipid components of exogenous sulphatide in human fibroblasts. BIOCHEMICAL JOURNAL, 252(2), 375-379.
Citazione: | Trinchera, M., Wiesmann, U., Pitto, M., Acquotti, D., & Ghidoni, R. (1988). Different metabolic recycling of the lipid components of exogenous sulphatide in human fibroblasts. BIOCHEMICAL JOURNAL, 252(2), 375-379. | |
Tipo: | Articolo in rivista - Articolo scientifico | |
Carattere della pubblicazione: | Scientifica | |
Presenza di un coautore afferente ad Istituzioni straniere: | No | |
Titolo: | Different metabolic recycling of the lipid components of exogenous sulphatide in human fibroblasts | |
Autori: | Trinchera, M; Wiesmann, U; Pitto, M; Acquotti, D; Ghidoni, R | |
Autori: | ||
Data di pubblicazione: | 1988 | |
Lingua: | English | |
Rivista: | BIOCHEMICAL JOURNAL | |
Appare nelle tipologie: | 01 - Articolo su rivista |