BACKGROUND: Immunophenotypic modulation is induced by steroids in pediatric B-cell precursor acute lymphoblastic leukemia (BCP-ALL) patients during remission induction therapy. METHODS: We cultured BCP-ALL blasts from diagnostic bone marrow (BM) samples (n = 20) in the presence of prednisone on stroma layer obtained from BM-derived mesenchymal cells to maintain viability. Antigen expression was assessed by multiparametric flow cytometry. RESULTS: Leukemia samples that sustained the treatment in vitro with prednisone, showed significative reduction of CD10 and CD34 expression compared with control, and it was comparable with that observed in residual leukemic cells of the same patients in BM at day 15 of treatment. Modulated cells were viable as determined by Annexin V staining and preserved light scattering properties. Of note, the extent of antigen modulation in vitro correlated with response to prednisone in vivo. CONCLUSIONS: The prednisone-induced immunophenotypic modulation can be reproduced in vitro and this phenomenon may reflect sensitivity to chemotherapy.

Gaipa, G., Basso, G., Aliprandi, S., Migliavacca, M., Vallinoto, C., Maglia, O., et al. (2008). Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B-cell precursor acute lymphoblastic leukemia cells. CYTOMETRY. PART B, CLINICAL CYTOMETRY, 74(3), 150-155 [10.1002/cyto.b.20408].

Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B-cell precursor acute lymphoblastic leukemia cells

FAINI, ANDREA;BIONDI, ANDREA;
2008

Abstract

BACKGROUND: Immunophenotypic modulation is induced by steroids in pediatric B-cell precursor acute lymphoblastic leukemia (BCP-ALL) patients during remission induction therapy. METHODS: We cultured BCP-ALL blasts from diagnostic bone marrow (BM) samples (n = 20) in the presence of prednisone on stroma layer obtained from BM-derived mesenchymal cells to maintain viability. Antigen expression was assessed by multiparametric flow cytometry. RESULTS: Leukemia samples that sustained the treatment in vitro with prednisone, showed significative reduction of CD10 and CD34 expression compared with control, and it was comparable with that observed in residual leukemic cells of the same patients in BM at day 15 of treatment. Modulated cells were viable as determined by Annexin V staining and preserved light scattering properties. Of note, the extent of antigen modulation in vitro correlated with response to prednisone in vivo. CONCLUSIONS: The prednisone-induced immunophenotypic modulation can be reproduced in vitro and this phenomenon may reflect sensitivity to chemotherapy.
Articolo in rivista - Articolo scientifico
Precursor Cells, B-Lymphoid; Adolescent; Male; Infant; Female; Child, Preschool; Cell Culture Techniques; Precursor B-Cell Lymphoblastic Leukemia-Lymphoma; Neprilysin; Humans; Flow Cytometry; Prednisone; Child; Antigens, CD34; Immunophenotyping; Remission Induction
English
mag-2008
74
3
150
155
none
Gaipa, G., Basso, G., Aliprandi, S., Migliavacca, M., Vallinoto, C., Maglia, O., et al. (2008). Prednisone induces immunophenotypic modulation of CD10 and CD34 in nonapoptotic B-cell precursor acute lymphoblastic leukemia cells. CYTOMETRY. PART B, CLINICAL CYTOMETRY, 74(3), 150-155 [10.1002/cyto.b.20408].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/14914
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