Assessment of minimal residual disease (MRD) by flow cytometry is considered to be based on the reproducibility of the leukemic immunophenotype detected at diagnosis. However, we previously noticed modulation of surface antigen expression in acute lymphoblastic leukemia (ALL) during the early treatment. Hence, we investigated this in 30 children with B-cell precursor ALL consecutively enrolled in the AIEOP-BFM ALL 2000 protocol. Quantitative expression of seven antigens useful in MRD monitoring was studied at diagnosis and compared to that measured at different time points of remission induction therapy. Downmodulation in the expression of CD10 and CD34 occurred at follow-up. By contrast, upmodulation of CD19, CD20, CD45RA, and CD11a was observed, while the expression of CD58 remained stable. Despite this, we could unambiguously discriminate leukemic cells from normal residual B cells. This holds true when bone marrow ( BM) samples from similarly treated T-ALL patients, but not from healthy donors, were used as reference. Our results indicate that immunophenotypic modulation occurs in ALL during the early phases of BFM-type protocols. However, the accuracy of MRD detection by flow cytometry seems not negatively affected if adequate analysis protocols are employed. Investigators should take this phenomenon into account in order to avoid pitfalls in flow cytometric MRD studies.

Gaipa, G., Basso, G., Maglia, O., Leoni, V., Faini, A., Cazzaniga, G., et al. (2005). Drug-induced immunophenotypic modulation in childhood ALL: implications for minimal residual disease detection. LEUKEMIA, 19(1), 49-56 [10.1038/sj.leu.2403559].

Drug-induced immunophenotypic modulation in childhood ALL: implications for minimal residual disease detection

FAINI, ANDREA;Cazzaniga, G;COLIVA, TIZIANA ANGELA;VALSECCHI, MARIA GRAZIA;BIONDI, ANDREA;
2005

Abstract

Assessment of minimal residual disease (MRD) by flow cytometry is considered to be based on the reproducibility of the leukemic immunophenotype detected at diagnosis. However, we previously noticed modulation of surface antigen expression in acute lymphoblastic leukemia (ALL) during the early treatment. Hence, we investigated this in 30 children with B-cell precursor ALL consecutively enrolled in the AIEOP-BFM ALL 2000 protocol. Quantitative expression of seven antigens useful in MRD monitoring was studied at diagnosis and compared to that measured at different time points of remission induction therapy. Downmodulation in the expression of CD10 and CD34 occurred at follow-up. By contrast, upmodulation of CD19, CD20, CD45RA, and CD11a was observed, while the expression of CD58 remained stable. Despite this, we could unambiguously discriminate leukemic cells from normal residual B cells. This holds true when bone marrow ( BM) samples from similarly treated T-ALL patients, but not from healthy donors, were used as reference. Our results indicate that immunophenotypic modulation occurs in ALL during the early phases of BFM-type protocols. However, the accuracy of MRD detection by flow cytometry seems not negatively affected if adequate analysis protocols are employed. Investigators should take this phenomenon into account in order to avoid pitfalls in flow cytometric MRD studies.
Articolo in rivista - Articolo scientifico
ALL; minimal residual disease; immunophenotype; flow cytometry; modulation
English
gen-2005
19
1
49
56
none
Gaipa, G., Basso, G., Maglia, O., Leoni, V., Faini, A., Cazzaniga, G., et al. (2005). Drug-induced immunophenotypic modulation in childhood ALL: implications for minimal residual disease detection. LEUKEMIA, 19(1), 49-56 [10.1038/sj.leu.2403559].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/14854
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