The recombinant enzymes uridine phosphorylase (UP) and purine nucleoside phosphorylase (PNP) were over-expressed in high-biomass bacterial fermentations and co-immobilized, without previous purification, on epoxy-activated solid supports by covalent linkages. These preparations are efficient biocatalysts of transglycosylation reactions and have been developed for producting natural and modified nucleosides of pharmaceutical interest in the field of antiviral and antitumoral agents. The new biocatalysts described in this work are suitable for both laboratory and industrial scale applications due to the maintainance of high catalytic efficiency, thermal and solvent stability, reusability and ease of operation in batch as well as in continuous reactions.

Zuffi, G., Ghisotti, D., Oliva, I., Capra, E., Frascotti, G., Tonon, G., et al. (2004). Immobilized biocatalysts for the production of nucleosides and nucleoside analogues by enzymatic transglycosylation reactions. BIOCATALYSIS AND BIOTRANSFORMATION, 22, 25-33 [10.1080/10242420310001648551].

Immobilized biocatalysts for the production of nucleosides and nucleoside analogues by enzymatic transglycosylation reactions

FRASCOTTI, GIANNI;
2004

Abstract

The recombinant enzymes uridine phosphorylase (UP) and purine nucleoside phosphorylase (PNP) were over-expressed in high-biomass bacterial fermentations and co-immobilized, without previous purification, on epoxy-activated solid supports by covalent linkages. These preparations are efficient biocatalysts of transglycosylation reactions and have been developed for producting natural and modified nucleosides of pharmaceutical interest in the field of antiviral and antitumoral agents. The new biocatalysts described in this work are suitable for both laboratory and industrial scale applications due to the maintainance of high catalytic efficiency, thermal and solvent stability, reusability and ease of operation in batch as well as in continuous reactions.
Articolo in rivista - Articolo scientifico
Scientifica
Immobilized biocatalysts; nucleosides and nucleoside analogues; enzymatic transglycosylation
English
Zuffi, G., Ghisotti, D., Oliva, I., Capra, E., Frascotti, G., Tonon, G., et al. (2004). Immobilized biocatalysts for the production of nucleosides and nucleoside analogues by enzymatic transglycosylation reactions. BIOCATALYSIS AND BIOTRANSFORMATION, 22, 25-33 [10.1080/10242420310001648551].
Zuffi, G; Ghisotti, D; Oliva, I; Capra, E; Frascotti, G; Tonon, G; Orsini, G
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10281/14679
Citazioni
  • Scopus 32
  • ???jsp.display-item.citation.isi??? 30
Social impact