PURPOSE: The PRL-3 phosphatase has been found expressed at higher levels in metastasis than in primary tumors of patients with colorectal cancer. In the present study, we evaluated the expression of PRL-3 in ovarian cancer tissue and its role in ovarian cancer cell growth. EXPERIMENTAL DESIGN: PRL-3 phosphatase expression was evaluated in 84 ovarian tumor samples. PRL-3 expression has been knocked down using specific small interfering RNAs to determine its role in ovarian cancer cell growth in vitro. RESULTS: In ovarian cancers, PRL-3 expression correlates with disease progression, being higher in advanced (stage III) than in early (stage I) tumors. In situ measurements of PRL-3 expression showed that it was confined to the epithelial neoplastic cells. The molecular mechanism underlying PRL-3 overexpression in ovarian cancers is independent from amplification of the corresponding genomic locus. Ovarian cancer cells growing in culture have high levels of expression of this phosphatase. PRL-3-specific knockdown using small interfering RNA severely impaired the growth of cells without affecting the expression of the closely related homologue PRL-1. Intriguingly, the growth of human colon carcinoma cells expressing lower levels of the PRL-3 was not affected by the PRL-3 knockdown. CONCLUSIONS: Altogether, these results show that PRL-3 expression is associated with ovarian cancer progression and point to a key role for this phosphatase in the control of ovarian cancer cells growth. This strongly suggests that PRL-3 should be considered as a target for the discovery of new anticancer agents to be tested against this malignancy.
Polato, F., Codegoni, A., Fruscio, R., Perego, P., Mangioni, C., Saha, S., et al. (2005). PRL-3 phosphatase is implicated in ovarian cancer growth. CLINICAL CANCER RESEARCH, 11(19 Pt 1), 6835-6839 [10.1158/1078-0432.CCR-04-2357].
|Citazione:||Polato, F., Codegoni, A., Fruscio, R., Perego, P., Mangioni, C., Saha, S., et al. (2005). PRL-3 phosphatase is implicated in ovarian cancer growth. CLINICAL CANCER RESEARCH, 11(19 Pt 1), 6835-6839 [10.1158/1078-0432.CCR-04-2357].|
|Tipo:||Articolo in rivista - Articolo scientifico|
|Carattere della pubblicazione:||Scientifica|
|Titolo:||PRL-3 phosphatase is implicated in ovarian cancer growth|
|Autori:||Polato, F; Codegoni, A; Fruscio, R; Perego, P; Mangioni, C; Saha, S; Bardelli, A; Broggini, M|
|Data di pubblicazione:||1-ott-2005|
|Rivista:||CLINICAL CANCER RESEARCH|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1158/1078-0432.CCR-04-2357|
|Appare nelle tipologie:||01 - Articolo su rivista|