Dendritic cells (DCs) are involved in the initiation and regulation of innate and adaptive immune responses. Several molecular mechanisms regulate these diverse DC functions, and we have previously reported that mouse dendritic cells (mDCs) can produce interleukin-2 (IL-2) in vitro and in vivo, in response to microbial activation and T-cell-mediated stimuli. This property is shared by different DC subtypes, including Langerhans cells. Here we show that, on appropriate stimulation, human DCs, both plasmacytoid and myeloid subtypes, also express IL-2. Interestingly, the production of IL-2 by myeloid DCs is induced by T-cell-mediated stimuli and depends on the presence of IL-15. The key role of this cytokine in regulating IL-2 production was also confirmed in the mouse system. In particular, we could show that DCs from IL-15-deficient mice were strongly impaired in the ability to produce IL-2 after interactions with different microbial stimuli. Our results indicate that DC-produced IL-2 is tightly coregulated with the expression of IL-15.

Feau, S., Facchinetti, V., Granucci, F., Citterio, S., Jarrossay, D., Seresini, S., et al. (2005). Dendritic cell-derived IL-2 production is regulated by IL-15 in humans and in mice. BLOOD, 105(2), 697-702 [10.1182/blood-2004-03-1059].

Dendritic cell-derived IL-2 production is regulated by IL-15 in humans and in mice

GRANUCCI, FRANCESCA;Citterio, S;CASTAGNOLI, PAOLA
2005

Abstract

Dendritic cells (DCs) are involved in the initiation and regulation of innate and adaptive immune responses. Several molecular mechanisms regulate these diverse DC functions, and we have previously reported that mouse dendritic cells (mDCs) can produce interleukin-2 (IL-2) in vitro and in vivo, in response to microbial activation and T-cell-mediated stimuli. This property is shared by different DC subtypes, including Langerhans cells. Here we show that, on appropriate stimulation, human DCs, both plasmacytoid and myeloid subtypes, also express IL-2. Interestingly, the production of IL-2 by myeloid DCs is induced by T-cell-mediated stimuli and depends on the presence of IL-15. The key role of this cytokine in regulating IL-2 production was also confirmed in the mouse system. In particular, we could show that DCs from IL-15-deficient mice were strongly impaired in the ability to produce IL-2 after interactions with different microbial stimuli. Our results indicate that DC-produced IL-2 is tightly coregulated with the expression of IL-15.
Articolo in rivista - Articolo scientifico
Cells, Cultured; Antigens, CD40; Interleukin-15; Mice, Knockout; Animals; Humans; Dendritic Cells; Interleukin-2; RNA, Messenger; Signal Transduction; Mice; Mice, Inbred C57BL; Monocytes; Mice, Inbred BALB C; Specific Pathogen-Free Organisms
English
15-gen-2005
105
2
697
702
none
Feau, S., Facchinetti, V., Granucci, F., Citterio, S., Jarrossay, D., Seresini, S., et al. (2005). Dendritic cell-derived IL-2 production is regulated by IL-15 in humans and in mice. BLOOD, 105(2), 697-702 [10.1182/blood-2004-03-1059].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/14461
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