A method based on the use of a Poroshell 120 EC-C18 column (3.0. mm. ×. 50. mm, 2.7. μm) with a high resolution electrospray ionization-quadrupole-time of flight (ESI-Q-ToF) tandem mass spectrometer as detection system was optimized for the identification of triacylglycerols (TAGs) in complex archaeological residues.The best performances in chromatographic separation and Q-ToF detection sensitivity were reached by using a mixture of iPrOH/MeOH at a 0.6. mL/min flow rate. Triolein (OOO) was eluted in 12.30. min and the chromatographic resolution against tripalmitin (PPP) was 1.4. Very good limits of detection and quantification limits were also achieved (LOD. <. 0.03. μg/g LOQ. <. 0.10. μg/g).These achievements were compared with the results provided by traditional particle columns and with the results reported in the recent literature regarding TAGs analysis in archaeological residues. Thanks to the unprecedented chromatographic separation and detection sensitivity attained, it was possible for the first time to perform TAGs researches in archaeological residues and reference materials with a dataset containing more than 500 molecular formula. © 2014 Elsevier B.V.

Saliu, F., Degano, I., Colombini, M. (2014). Identification of triacylglycerols in archaelogical organic residues by core-shell reversed phase liquid chromatography coupled to electrospray ionization-quadrupole-time of flight mass spectrometry. JOURNAL OF CHROMATOGRAPHY A, 1346, 78-87 [10.1016/j.chroma.2014.04.049].

Identification of triacylglycerols in archaelogical organic residues by core-shell reversed phase liquid chromatography coupled to electrospray ionization-quadrupole-time of flight mass spectrometry

SALIU, FRANCESCO
;
2014

Abstract

A method based on the use of a Poroshell 120 EC-C18 column (3.0. mm. ×. 50. mm, 2.7. μm) with a high resolution electrospray ionization-quadrupole-time of flight (ESI-Q-ToF) tandem mass spectrometer as detection system was optimized for the identification of triacylglycerols (TAGs) in complex archaeological residues.The best performances in chromatographic separation and Q-ToF detection sensitivity were reached by using a mixture of iPrOH/MeOH at a 0.6. mL/min flow rate. Triolein (OOO) was eluted in 12.30. min and the chromatographic resolution against tripalmitin (PPP) was 1.4. Very good limits of detection and quantification limits were also achieved (LOD. <. 0.03. μg/g LOQ. <. 0.10. μg/g).These achievements were compared with the results provided by traditional particle columns and with the results reported in the recent literature regarding TAGs analysis in archaeological residues. Thanks to the unprecedented chromatographic separation and detection sensitivity attained, it was possible for the first time to perform TAGs researches in archaeological residues and reference materials with a dataset containing more than 500 molecular formula. © 2014 Elsevier B.V.
Articolo in rivista - Articolo scientifico
Archaeological residues; Core-shell stationary phase; Liquid chromatography-electrospray ionization-quadrupole-time of flight mass spectrometry (HPLC-ESI-Q-ToF); Triacyglycerols (TAGs); Archaeology; Ointments; Reproducibility of Results; Sensitivity and Specificity; Triglycerides; Chromatography, Reverse-Phase; Spectrometry, Mass, Electrospray Ionization; Analytical Chemistry; Organic Chemistry; Biochemistry
English
78
87
10
Saliu, F., Degano, I., Colombini, M. (2014). Identification of triacylglycerols in archaelogical organic residues by core-shell reversed phase liquid chromatography coupled to electrospray ionization-quadrupole-time of flight mass spectrometry. JOURNAL OF CHROMATOGRAPHY A, 1346, 78-87 [10.1016/j.chroma.2014.04.049].
Saliu, F; Degano, I; Colombini, M
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/140866
Citazioni
  • Scopus 21
  • ???jsp.display-item.citation.isi??? 19
Social impact