Antineutrophil cytoplasmic antibodies (ANCA) directed against proteinase 3 (PR3) are a sensitive and specific marker for ANCA-associated systemic vasculitis and, in particular, for Wegener's granulomatosis (WG). Using indirect immunofluorescence (IIF) on ethanol-fixed neutrophils, antibodies to PR3 give a cytoplasmic staining (CANCA). They are usually detected by Enzyme-linked immunosorbent assay (ELISA) using purified PR3 as substrate (direct ELISA). An alternative and promising method is constituted by a capture ELISA, where a monoclonal antibody to PR3 is used as capturing ligand. The best diagnostic performance is obtained when IIF is combined with PR3-specific ELISA. PR3-ANCA are present in about 65% of patients with WG and in a much lower percentage of patients with microscopic polyangiitis (and Churg-Strauss syndrome). Their prevalence is influenced by disease activity/extent and by the methodology used. PR3-ANCA levels are useful to monitor disease activity but should not be used by themselves to guide treatment. A significant increase in ANCA titres, or the reappearance of ANCA, should alert the clinicians and lead to a stricter patient control. There is increasing, mainly indirect, evidence that ANCA may have a pathogenic role. © 2007 Elsevier Inc. All rights reserved.

Radice, A., Sabadini, E., Sinico, R. (2007). Antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3. In Autoantibodies (pp. 105-110). Elsevier Inc. [10.1016/B978-044452763-9/50018-4].

Antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3

SINICO, RENATO ALBERTO
2007

Abstract

Antineutrophil cytoplasmic antibodies (ANCA) directed against proteinase 3 (PR3) are a sensitive and specific marker for ANCA-associated systemic vasculitis and, in particular, for Wegener's granulomatosis (WG). Using indirect immunofluorescence (IIF) on ethanol-fixed neutrophils, antibodies to PR3 give a cytoplasmic staining (CANCA). They are usually detected by Enzyme-linked immunosorbent assay (ELISA) using purified PR3 as substrate (direct ELISA). An alternative and promising method is constituted by a capture ELISA, where a monoclonal antibody to PR3 is used as capturing ligand. The best diagnostic performance is obtained when IIF is combined with PR3-specific ELISA. PR3-ANCA are present in about 65% of patients with WG and in a much lower percentage of patients with microscopic polyangiitis (and Churg-Strauss syndrome). Their prevalence is influenced by disease activity/extent and by the methodology used. PR3-ANCA levels are useful to monitor disease activity but should not be used by themselves to guide treatment. A significant increase in ANCA titres, or the reappearance of ANCA, should alert the clinicians and lead to a stricter patient control. There is increasing, mainly indirect, evidence that ANCA may have a pathogenic role. © 2007 Elsevier Inc. All rights reserved.
Capitolo o saggio
Biochemistry, Genetics and Molecular Biology (all)
English
Autoantibodies
2007
9780444527639
Radice, A., Sabadini, E., Sinico, R. (2007). Antineutrophil cytoplasmic autoantibodies with specificity for proteinase 3. In Autoantibodies (pp. 105-110). Elsevier Inc. [10.1016/B978-044452763-9/50018-4].
Radice, A; Sabadini, E; Sinico, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/140388
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