Peripheral blood mononuclear cells (PBMC) from patients with Essential Mixed Cryoglobulinemia (EMC) were studied for their ability to synthesize polyclonal IgM and rheumatoid factor (RF) IgM in vitro. Our results indicate: that EMC-PBMC produce smaller amounts of polyclonal IgM but higher quantities of IgM-RF than normal PBMC after pokeweed mitogen (PWM) or Staphylococcus aureus activation, so that the IgM-RF to total IgM ratio is significantly greater in EMC than in normal cultures; that enriched EMC-B lymphocytes display a significantly higher spontaneous synthesis of IgM-RF than normal B lymphocytes and that the IgM-RF B cell clones are receptive to T cell regulation. Taken together these findings suggest an expansion of B cell clones committed to IgM RF production and the presence in peripheral blood of differentiated B lymphocytes capable of secreting IgM-RF in EMC

Meroni, P., Barcellini, W., Sinico, R., Fornasieri, A., Sguotti, C., Invernizzi, F., et al. (1986). In vitro synthesis of IgM rheumatoid factor by lymphocytes from patients with essential mixed cryoglobulinemia. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 65(2), 303-310.

In vitro synthesis of IgM rheumatoid factor by lymphocytes from patients with essential mixed cryoglobulinemia

SINICO, RENATO ALBERTO;
1986

Abstract

Peripheral blood mononuclear cells (PBMC) from patients with Essential Mixed Cryoglobulinemia (EMC) were studied for their ability to synthesize polyclonal IgM and rheumatoid factor (RF) IgM in vitro. Our results indicate: that EMC-PBMC produce smaller amounts of polyclonal IgM but higher quantities of IgM-RF than normal PBMC after pokeweed mitogen (PWM) or Staphylococcus aureus activation, so that the IgM-RF to total IgM ratio is significantly greater in EMC than in normal cultures; that enriched EMC-B lymphocytes display a significantly higher spontaneous synthesis of IgM-RF than normal B lymphocytes and that the IgM-RF B cell clones are receptive to T cell regulation. Taken together these findings suggest an expansion of B cell clones committed to IgM RF production and the presence in peripheral blood of differentiated B lymphocytes capable of secreting IgM-RF in EMC
Articolo in rivista - Articolo scientifico
Adult; Aged; B-Lymphocytes; Cryoglobulinemia; Female; Humans; Immunoglobulin M; Lymphocyte Activation; Lymphocytes; Male; Middle Aged; Mitogens; Rheumatoid Factor; T-Lymphocytes
English
303
310
8
Meroni, P., Barcellini, W., Sinico, R., Fornasieri, A., Sguotti, C., Invernizzi, F., et al. (1986). In vitro synthesis of IgM rheumatoid factor by lymphocytes from patients with essential mixed cryoglobulinemia. CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 65(2), 303-310.
Meroni, P; Barcellini, W; Sinico, R; Fornasieri, A; Sguotti, C; Invernizzi, F; D'Amico, G; Zanussi, C
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/140270
Citazioni
  • Scopus 10
  • ???jsp.display-item.citation.isi??? 16
Social impact