A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was used to detect IgA rheumatoid factor (RF) in sera from 88 patients with IgA nephropathy (IgA GN), a disease characterized by abnormalities of IgA production. Significantly higher levels of IgA antiglobulins were demonstrated in IgA GN patients than in normal healthy controls and patients with other forms of chronic primary glomerulonephritis (mean +/- SEM 28.4 +/- 6.6 vs 6.0 +/- 0.4 and 8.3 +/- 1.2 micrograms/ml respectively; p less than 0.002). Interestingly, in contrast to rheumatoid arthritis, IgA RF activity was not associated with IgM antiglobulins. Analysis of sera fractionated by gel chromatography at acid pH revealed that anti-IgG activity resided predominantly in the polymeric fractions of IgA as confirmed by the ability to bind "free" secretory component. Several findings in patients with IgA GN suggest that the IgA deposited in the glomeruli is polymeric, and levels of circulating macromolecular IgA are increased. Our findings confirm a general perturbation of IgA metabolism in this disease. Although the polymeric nature of the IgA RF is suggestive of a mucosal origin, additional evidence is needed to confirm this hypothesis

Sinico, R., Fornasieri, A., Oreni, N., Benuzzi, S., & D'Amico, G. (1986). Polymeric IgA rheumatoid factor in idiopathic IgA mesangial nephropathy (Berger's disease). JOURNAL OF IMMUNOLOGY, 137(2), 536-541.

Polymeric IgA rheumatoid factor in idiopathic IgA mesangial nephropathy (Berger's disease)

SINICO, RENATO ALBERTO
Primo
;
1986

Abstract

A specific and sensitive enzyme-linked immunosorbent assay (ELISA) was used to detect IgA rheumatoid factor (RF) in sera from 88 patients with IgA nephropathy (IgA GN), a disease characterized by abnormalities of IgA production. Significantly higher levels of IgA antiglobulins were demonstrated in IgA GN patients than in normal healthy controls and patients with other forms of chronic primary glomerulonephritis (mean +/- SEM 28.4 +/- 6.6 vs 6.0 +/- 0.4 and 8.3 +/- 1.2 micrograms/ml respectively; p less than 0.002). Interestingly, in contrast to rheumatoid arthritis, IgA RF activity was not associated with IgM antiglobulins. Analysis of sera fractionated by gel chromatography at acid pH revealed that anti-IgG activity resided predominantly in the polymeric fractions of IgA as confirmed by the ability to bind "free" secretory component. Several findings in patients with IgA GN suggest that the IgA deposited in the glomeruli is polymeric, and levels of circulating macromolecular IgA are increased. Our findings confirm a general perturbation of IgA metabolism in this disease. Although the polymeric nature of the IgA RF is suggestive of a mucosal origin, additional evidence is needed to confirm this hypothesis
Articolo in rivista - Articolo scientifico
Adolescent; Adult; Aged; Antibody Specificity; Child; Chromatography, Affinity; Chromatography, Gel; Enzyme-Linked Immunosorbent Assay; Female; Glomerulonephritis, IGA; Humans; Immunoglobulin A; Male; Middle Aged; Molecular Weight; Rheumatoid Factor
English
536
541
6
Sinico, R., Fornasieri, A., Oreni, N., Benuzzi, S., & D'Amico, G. (1986). Polymeric IgA rheumatoid factor in idiopathic IgA mesangial nephropathy (Berger's disease). JOURNAL OF IMMUNOLOGY, 137(2), 536-541.
Sinico, R; Fornasieri, A; Oreni, N; Benuzzi, S; D'Amico, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/140268
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