Aim: We aimed to establish a 3D vascularized in vitro bone remodeling model. Materials & methods: Human umbilical endothelial cells (HUVECs), bone marrow mesenchymal stem cells (BMSCs), and osteoblast (OBs) and osteoclast (OCs) precursors were embedded in collagen/fibrin hydrogels enriched with calcium phosphate nanoparticles (CaPn). We assessed vasculogenesis in HUVEC-BMSC coculture, osteogenesis with OBs, osteoclastogenesis with OCs, and, ultimately, cell interplay in tetraculture. Results: HUVECs developed a robust microvascular network and BMSCs differentiated into mural cells. Noteworthy, OB and OC differentiation was increased by their reciprocal coculture and by CaPn, and even more by the combination of the tetraculture and CaPn. Conclusion: We successfully developed a vascularized 3D bone remodeling model, whereby cells interacted and exerted their specific function.

Bongio, M., Lopa, S., Gilardi, M., Bersini, S., Moretti, M. (2016). A 3D vascularized bone remodeling model combining osteoblasts and osteoclasts in a CaP nanoparticle-enriched matrix. NANOMEDICINE, 11(9), 1073-1091 [10.2217/nnm-2015-0021].

A 3D vascularized bone remodeling model combining osteoblasts and osteoclasts in a CaP nanoparticle-enriched matrix

GILARDI, MARA;
2016

Abstract

Aim: We aimed to establish a 3D vascularized in vitro bone remodeling model. Materials & methods: Human umbilical endothelial cells (HUVECs), bone marrow mesenchymal stem cells (BMSCs), and osteoblast (OBs) and osteoclast (OCs) precursors were embedded in collagen/fibrin hydrogels enriched with calcium phosphate nanoparticles (CaPn). We assessed vasculogenesis in HUVEC-BMSC coculture, osteogenesis with OBs, osteoclastogenesis with OCs, and, ultimately, cell interplay in tetraculture. Results: HUVECs developed a robust microvascular network and BMSCs differentiated into mural cells. Noteworthy, OB and OC differentiation was increased by their reciprocal coculture and by CaPn, and even more by the combination of the tetraculture and CaPn. Conclusion: We successfully developed a vascularized 3D bone remodeling model, whereby cells interacted and exerted their specific function.
Articolo in rivista - Articolo scientifico
3D model; bone remodeling; vascularization;
3D model; bone remodeling; vascularization; Bone Marrow; Bone Remodeling; Calcium Phosphates; Cell Differentiation; Cell Proliferation; Coculture Techniques; Human Umbilical Vein Endothelial Cells; Humans; Hydrogels; Mesenchymal Stromal Cells; Nanoparticles; Osteoblasts; Osteoclasts; Osteogenesis
English
2016
11
9
1073
1091
none
Bongio, M., Lopa, S., Gilardi, M., Bersini, S., Moretti, M. (2016). A 3D vascularized bone remodeling model combining osteoblasts and osteoclasts in a CaP nanoparticle-enriched matrix. NANOMEDICINE, 11(9), 1073-1091 [10.2217/nnm-2015-0021].
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/139618
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