Recently, the induction of SLE in naive mice employing monoclonal anti-DNA antibodies (anti-DNA Ab) carrying the pathogenic idiotype 16/6 (16/6 Id) has been reported. In the current study we report on the induction of experimental SLE by polyclonal IgG anti-DNA Ab derived from a patient with active SLE and carrying the 16/6 Id. Two different experiments were conducted in which BALB/c mice were immunized in the footpads with 1 microgram/ml or 5 micrograms/ml of anti-DNA Ab. The first experiment showed the appearance in the immunized mice of high titre anti-DNA Ab together with antinuclear antibodies, alopecia and proteinuria. In the second experiment we compared, as immunizing agents, 16/6 positive anti-DNA Ab and 16/6 negative anti-tetanus toxoid antibodies (anti-TT Ab) obtained from the serum of the same patients. Our results show that only mice immunized with 16/6 positive antibodies produced anti-DNA Ab, while mice immunized with anti-TT Ab did not show any DNA-binding activity but, surprisingly, developed high titre anti-cardiolipin antibodies

Tincani, A., Balestrieri, G., Allegri, F., Cattaneo, R., Fornasieri, A., Li, M., et al. (1993). Induction of experimental SLE in naive mice by immunization with human polyclonal anti-DNA antibody carrying the 16/6 idiotype. CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, 11(2), 129-134.

Induction of experimental SLE in naive mice by immunization with human polyclonal anti-DNA antibody carrying the 16/6 idiotype

SINICO, RENATO ALBERTO
Penultimo
;
1993

Abstract

Recently, the induction of SLE in naive mice employing monoclonal anti-DNA antibodies (anti-DNA Ab) carrying the pathogenic idiotype 16/6 (16/6 Id) has been reported. In the current study we report on the induction of experimental SLE by polyclonal IgG anti-DNA Ab derived from a patient with active SLE and carrying the 16/6 Id. Two different experiments were conducted in which BALB/c mice were immunized in the footpads with 1 microgram/ml or 5 micrograms/ml of anti-DNA Ab. The first experiment showed the appearance in the immunized mice of high titre anti-DNA Ab together with antinuclear antibodies, alopecia and proteinuria. In the second experiment we compared, as immunizing agents, 16/6 positive anti-DNA Ab and 16/6 negative anti-tetanus toxoid antibodies (anti-TT Ab) obtained from the serum of the same patients. Our results show that only mice immunized with 16/6 positive antibodies produced anti-DNA Ab, while mice immunized with anti-TT Ab did not show any DNA-binding activity but, surprisingly, developed high titre anti-cardiolipin antibodies
Articolo in rivista - Articolo scientifico
Alopecia; Animals; Antibodies, Anticardiolipin; Antibodies, Antinuclear; Counterimmunoelectrophoresis; DNA; Disease Models, Animal; Enzyme-Linked Immunosorbent Assay; Epitopes; Female; Fluorescent Antibody Technique; Immunization, Passive; Immunoglobulin Idiotypes; Lupus Erythematosus, Systemic; Mice; Mice, Inbred BALB C; Proteinuria
English
129
134
6
Tincani, A., Balestrieri, G., Allegri, F., Cattaneo, R., Fornasieri, A., Li, M., et al. (1993). Induction of experimental SLE in naive mice by immunization with human polyclonal anti-DNA antibody carrying the 16/6 idiotype. CLINICAL AND EXPERIMENTAL RHEUMATOLOGY, 11(2), 129-134.
Tincani, A; Balestrieri, G; Allegri, F; Cattaneo, R; Fornasieri, A; Li, M; Sinico, R; D'Amico, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/139496
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