To verify whether IgA antineutrophil cytoplasmic antibody (ANCA) represents a serologic marker in Henoch-Schönlein purpura (HPS), we examined sera from 41 patients with the disease. Control sera from 28 patients with primary IgA nephropathy (IgA-N), 26 IgG-ANCA-positive vasculitis, and 28 normal controls were also studied. An increased IgA binding to neutrophil cytoplasmic extracts but not to purified ANCA antigens was found in 12.2-14.6% of HSP patients and in 14.3-21.4% of IgA-N patients versus 3.5% of normal controls. IgA binding to neutrophil cytoplasmic extracts correlated with serum IgA levels, IgA-rheumatoid factor, and IgA-fibronectin binding capacity. Moreover, low amounts of IgG and fibronectin were detected as contaminants in neutrophil cytoplasmic extracts and fibronectin could partly inhibit the binding of IgA to "crude" extracts. We conclude that IgA-ANCA are neither diagnostically nor immunologically specific in HSP and IgA-N. Several factors present in the sera of patients with IgA-related nephropathies seem to contribute to the "false-positive" IgA-ANCA demonstrable in these patients

Sinico, R., Tadros, M., Radice, A., Pozzi, C., Quarenghi, M., Comotti, C., et al. (1994). Lack of IgA antineutrophil cytoplasmic antibodies in Henoch-Schönlein purpura and IgA nephropathy. CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 73(1), 19-26 [10.1006/clin.1994.1165].

Lack of IgA antineutrophil cytoplasmic antibodies in Henoch-Schönlein purpura and IgA nephropathy

SINICO, RENATO ALBERTO
Primo
;
1994

Abstract

To verify whether IgA antineutrophil cytoplasmic antibody (ANCA) represents a serologic marker in Henoch-Schönlein purpura (HPS), we examined sera from 41 patients with the disease. Control sera from 28 patients with primary IgA nephropathy (IgA-N), 26 IgG-ANCA-positive vasculitis, and 28 normal controls were also studied. An increased IgA binding to neutrophil cytoplasmic extracts but not to purified ANCA antigens was found in 12.2-14.6% of HSP patients and in 14.3-21.4% of IgA-N patients versus 3.5% of normal controls. IgA binding to neutrophil cytoplasmic extracts correlated with serum IgA levels, IgA-rheumatoid factor, and IgA-fibronectin binding capacity. Moreover, low amounts of IgG and fibronectin were detected as contaminants in neutrophil cytoplasmic extracts and fibronectin could partly inhibit the binding of IgA to "crude" extracts. We conclude that IgA-ANCA are neither diagnostically nor immunologically specific in HSP and IgA-N. Several factors present in the sera of patients with IgA-related nephropathies seem to contribute to the "false-positive" IgA-ANCA demonstrable in these patients
Articolo in rivista - Articolo scientifico
Adolescent; Adult; Antibodies, Antineutrophil Cytoplasmic; Autoantibodies; Child; Child, Preschool; Enzyme-Linked Immunosorbent Assay; Fibronectins; Glomerulonephritis, IGA; Granulocytes; Humans; Immunoglobulin A; Infant; Infant, Newborn; Purpura, Schoenlein-Henoch; Rheumatoid Factor
English
19
26
8
Sinico, R., Tadros, M., Radice, A., Pozzi, C., Quarenghi, M., Comotti, C., et al. (1994). Lack of IgA antineutrophil cytoplasmic antibodies in Henoch-Schönlein purpura and IgA nephropathy. CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY, 73(1), 19-26 [10.1006/clin.1994.1165].
Sinico, R; Tadros, M; Radice, A; Pozzi, C; Quarenghi, M; Comotti, C; Gregorini, G; Castiglione, A; Arrigo, G; D'Amico, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/139482
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