Background. The role of anti-glomerular basement membrane (GBM) antibodies in the pathogenesis of Goodpasture syndrome (GPS) is firmly established. Untreated, the disease may follow a fulminating course. Early identification of patients has important implications in terms of management and prognosis. Therefore, a diagnostic test for the determination of circulating anti-GBM antibodies, of very high sensitivity and specificity, is necessary. A number of assays, using different antigenic substrates, are available, but studies comparing the 'performances' of the different tests are scarce. Methods. The aim of our work was to evaluate the sensitivity and specificity of four immunoassay-based anti-GBM antibodies kits. Thirty-four serum samples from 19 GPS patients, 41 pathological and 28 normal controls were studied retrospectively (the follow-up samples were not included in the analysis of performance data). Cut-off limits were derived from receiver operating characteristics curve analysis. Results. All the assays showed a comparable good sensitivity (between 94.7 and 100.0%), whereas specificity varied considerably (from 90.9 to 100.0%). The better performance in terms of sensitivity/specificity was achieved by a fluorescence immunoassay which utilizes a recombinant antigen. Conclusion. All the assays have a good performance, with high sensitivity; however, the specificity may vary considerably. © The Author [2005]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved

Sinico, R., Radice, A., Corace, C., Sabadini, E., Bollini, B. (2006). Anti-glomerular basement membrane antibodies in the diagnosis of Goodpasture syndrome: A comparison of different assays. NEPHROLOGY DIALYSIS TRANSPLANTATION, 21(2), 397-401 [10.1093/ndt/gfi230].

Anti-glomerular basement membrane antibodies in the diagnosis of Goodpasture syndrome: A comparison of different assays

SINICO, RENATO ALBERTO
;
2006

Abstract

Background. The role of anti-glomerular basement membrane (GBM) antibodies in the pathogenesis of Goodpasture syndrome (GPS) is firmly established. Untreated, the disease may follow a fulminating course. Early identification of patients has important implications in terms of management and prognosis. Therefore, a diagnostic test for the determination of circulating anti-GBM antibodies, of very high sensitivity and specificity, is necessary. A number of assays, using different antigenic substrates, are available, but studies comparing the 'performances' of the different tests are scarce. Methods. The aim of our work was to evaluate the sensitivity and specificity of four immunoassay-based anti-GBM antibodies kits. Thirty-four serum samples from 19 GPS patients, 41 pathological and 28 normal controls were studied retrospectively (the follow-up samples were not included in the analysis of performance data). Cut-off limits were derived from receiver operating characteristics curve analysis. Results. All the assays showed a comparable good sensitivity (between 94.7 and 100.0%), whereas specificity varied considerably (from 90.9 to 100.0%). The better performance in terms of sensitivity/specificity was achieved by a fluorescence immunoassay which utilizes a recombinant antigen. Conclusion. All the assays have a good performance, with high sensitivity; however, the specificity may vary considerably. © The Author [2005]. Published by Oxford University Press on behalf of ERA-EDTA. All rights reserved
Articolo in rivista - Articolo scientifico
Anti-GBM disease; Anti-glomerular basement membrane (GBM) antibodies; ELISA; Goodpasture syndrome; NC1 portion of the α3 chain of type IV collagen; Anti-Glomerular Basement Membrane Disease; Antibodies; Autoantibodies; Humans; Immunoassay; Sensitivity and Specificity; Nephrology; Transplantation
English
2006
21
2
397
401
none
Sinico, R., Radice, A., Corace, C., Sabadini, E., Bollini, B. (2006). Anti-glomerular basement membrane antibodies in the diagnosis of Goodpasture syndrome: A comparison of different assays. NEPHROLOGY DIALYSIS TRANSPLANTATION, 21(2), 397-401 [10.1093/ndt/gfi230].
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10281/139404
Citazioni
  • Scopus 96
  • ???jsp.display-item.citation.isi??? 77
Social impact