Three different yeast strains, namely Saccharomyces cerevisiae mnn1mnn9, Kluyveromyces lactis JA6/GAA and Zygosaccharomyces bailii [pZ <sub>3</sub>klIL-1β], were entrapped in polyvinyl alcohol (PVA) gel particles, obtained following the commercially available immobilization kit named Lentikat®. After immobilization in the PVA-gel particles, yeast cells remained viable: colonization of the gel matrix reached up 100 mg d.w. of cells cm<sup>-3</sup> gel for all the strains examined. Lentikats® of K. lactis JA6/GAA and Z. bailii [pZ<sub>3</sub>klIL-1β] showed to be suitable for the continuous production of glucoamylase and interleukin 1β, respectively, when employed under non-selective conditions. They were of easy handiness and showed excellent mechanical properties during prolonged operation in stirred tank reactors. © 2005 Elsevier Inc. All rights reserved.
Parascandola, P., Branduardi, P., De Alteriis, E. (2006). PVA-gel (Lentikats®) as an effective matrix for yeast strain immobilization aimed at heterologous protein production. ENZYME AND MICROBIAL TECHNOLOGY, 38(1-2), 184-189 [10.1016/j.enzmictec.2005.06.004].
PVA-gel (Lentikats®) as an effective matrix for yeast strain immobilization aimed at heterologous protein production
BRANDUARDI, PAOLA;
2006
Abstract
Three different yeast strains, namely Saccharomyces cerevisiae mnn1mnn9, Kluyveromyces lactis JA6/GAA and Zygosaccharomyces bailii [pZ 3klIL-1β], were entrapped in polyvinyl alcohol (PVA) gel particles, obtained following the commercially available immobilization kit named Lentikat®. After immobilization in the PVA-gel particles, yeast cells remained viable: colonization of the gel matrix reached up 100 mg d.w. of cells cm-3 gel for all the strains examined. Lentikats® of K. lactis JA6/GAA and Z. bailii [pZ3klIL-1β] showed to be suitable for the continuous production of glucoamylase and interleukin 1β, respectively, when employed under non-selective conditions. They were of easy handiness and showed excellent mechanical properties during prolonged operation in stirred tank reactors. © 2005 Elsevier Inc. All rights reserved.
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